Cellular Prion Protein Expression Is Not Regulated by the Alzheimer's Amyloid Precursor Protein Intracellular Domain

Victoria Lewis*, Isobel J. Whitehouse, Herbert Baybutt, Jean C. Manson, Steven J. Collins, Nigel M. Hooper

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

There is increasing evidence of molecular and cellular links between Alzheimer's disease (AD) and prion diseases. The cellular prion protein, PrPC, modulates the posttranslational processing of the AD amyloid precursor protein (APP), through its inhibition of the beta-secretase BACE1, and oligomers of amyloid-beta bind to PrPC which may mediate amyloid-beta neurotoxicity. In addition, the APP intracellular domain (AICD), which acts as a transcriptional regulator, has been reported to control the expression of PrPC. Through the use of transgenic mice, cell culture models and manipulation of APP expression and processing, this study aimed to clarify the role of AICD in regulating PrPC. Overexpression of the three major isoforms of human APP (APP(695), APP(751) and APP(770)) in cultured neuronal and non-neuronal cells had no effect on the level of endogenous PrPC. Furthermore, analysis of brain tissue from transgenic mice over-expressing either wild type or familial AD associated mutant human APP revealed unaltered PrPC levels. Knockdown of endogenous APP expression in cells by siRNA or inhibition of gamma-secretase activity also had no effect on PrPC levels. Overall, we did not detect any significant difference in the expression of PrPC in any of the cell or animal-based paradigms considered, indicating that the control of cellular PrPC levels by AICD is not as straightforward as previously suggested.

Original languageEnglish
Article numberARTN e31754
Number of pages8
JournalPLoS ONE
Volume7
Issue number2
DOIs
Publication statusPublished - 21 Feb 2012

Keywords

  • GENE
  • APP
  • COEXISTENCE
  • IMPAIRMENT
  • NEPRILYSIN
  • CREUTZFELDT-JAKOB-DISEASE
  • BETA OLIGOMERS
  • TRANSCRIPTIONAL CONTROL
  • WILD-TYPE
  • MECHANISMS

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