Cellular uptake of fluorescent labelled biotin-streptavidin microspheres

M. Bradley, L. Alexander, R. M. Sanchez-Martin

Research output: Contribution to journalArticlepeer-review

Abstract

Amino functionalized, cross-linked, polystyrene microspheres were covalently loaded with streptavidin to which was coupled fluorescently labeled biotin and biotinylated-tagged DNA. These biotin-streptavidin microsphere conjugates were then successfully delivered into cells. The application of the streptavidin-biotin technology to these microspheres allows the effective delivery of any biotinylated material into intact mammalian cells, without the need for delicate procedures such as micro-injection.

Original languageEnglish
Pages (from-to)733-739
Number of pages7
JournalJournal of fluorescence
Volume18
Issue number3-4
DOIs
Publication statusPublished - Jul 2008

Keywords

  • multifunctionalised microspheres
  • streptavidin-biotin
  • flow cytometry
  • fluorescent imaging
  • intracellular delivery
  • PENETRATING PEPTIDES
  • MAMMALIAN-CELLS
  • DRUG-DELIVERY
  • DENDRIMERS
  • PROTEIN
  • AVIDIN

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