Abstract / Description of output
Amino functionalized, cross-linked, polystyrene microspheres were covalently loaded with streptavidin to which was coupled fluorescently labeled biotin and biotinylated-tagged DNA. These biotin-streptavidin microsphere conjugates were then successfully delivered into cells. The application of the streptavidin-biotin technology to these microspheres allows the effective delivery of any biotinylated material into intact mammalian cells, without the need for delicate procedures such as micro-injection.
Original language | English |
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Pages (from-to) | 733-739 |
Number of pages | 7 |
Journal | Journal of fluorescence |
Volume | 18 |
Issue number | 3-4 |
DOIs | |
Publication status | Published - Jul 2008 |
Keywords / Materials (for Non-textual outputs)
- multifunctionalised microspheres
- streptavidin-biotin
- flow cytometry
- fluorescent imaging
- intracellular delivery
- PENETRATING PEPTIDES
- MAMMALIAN-CELLS
- DRUG-DELIVERY
- DENDRIMERS
- PROTEIN
- AVIDIN