CENP-A confers a reduction in height on octameric nucleosomes

M.D.D. Miell, C.J. Fuller, A. Guse, H.M. Barysz, A. Downes, T. Owen-Hughes, J. Rappsilber, A.F. Straight, R.C. Allshire

Research output: Contribution to journalArticlepeer-review

Abstract

Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.
Original languageEnglish
Pages (from-to)763-765
Number of pages3
JournalNature Structural & Molecular Biology
Volume20
Issue number6
Early online date5 May 2013
DOIs
Publication statusPublished - 1 Jun 2013

Keywords

  • atomic force microscopy, nuclesomes, histones, CENP-A.

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