CENP-A confers a reduction in height on octameric nucleosomes

M.D.D. Miell; C.J. Fuller; A. Guse; H.M. Barysz; A. Downes; T. Owen-Hughes; J. Rappsilber; A.F. Straight; R.C. Allshire

doi:10.1038/nsmb.2574

CENP-A confers a reduction in height on octameric nucleosomes

M.D.D. Miell, C.J. Fuller, A. Guse, H.M. Barysz, A. Downes, T. Owen-Hughes, J. Rappsilber, A.F. Straight, R.C. Allshire

Research output: Contribution to journal › Article › peer-review

Abstract

Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

Original language	English
Pages (from-to)	763-765
Number of pages	3
Journal	Nature Structural & Molecular Biology
Volume	20
Issue number	6
Early online date	5 May 2013
DOIs	https://doi.org/10.1038/nsmb.2574
Publication status	Published - 1 Jun 2013

Keywords / Materials (for Non-textual outputs)

atomic force microscopy, nuclesomes, histones, CENP-A.

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abstract = "Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.",

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AU - Miell, M.D.D.

AU - Fuller, C.J.

AU - Guse, A.

AU - Barysz, H.M.

AU - Downes, A.

AU - Owen-Hughes, T.

AU - Rappsilber, J.

AU - Straight, A.F.

AU - Allshire, R.C.

PY - 2013/6/1

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N2 - Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

AB - Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

KW - atomic force microscopy, nuclesomes, histones, CENP-A.

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DO - 10.1038/nsmb.2574

M3 - Article

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JO - Nature Structural & Molecular Biology

JF - Nature Structural & Molecular Biology

IS - 6

ER -

CENP-A confers a reduction in height on octameric nucleosomes

Abstract

Keywords / Materials (for Non-textual outputs)

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