Centrosome docking at the immunological synapse is controlled by Lck signaling

Andy Tsun, Ihjaaz Qureshi, Jane C. Stinchcombe, Misty R. Jenkins, Maike de la Roche, Joanna Kleczkowska, Rose Zamoyska, Gillian M. Griffiths

Research output: Contribution to journalArticlepeer-review

Abstract

Docking of the centrosome at the plasma membrane directs lytic granules to the immunological synapse. To identify signals controlling centrosome docking at the synapse, we have studied cytotoxic T lymphocytes (CTLs) in which expression of the T cell receptor-activated tyrosine kinase Lck is ablated. In the absence of Lck, the centrosome is able to translocate around the nucleus toward the immunological synapse but is unable to dock at the plasma membrane. Lytic granules fail to polarize and release their contents, and target cells are not killed. In CTLs deficient in both Lck and the related tyrosine kinase Fyn, centrosome translocation is impaired, and the centrosome remains on the distal side of the nucleus relative to the synapse. These results show that repositioning of the centrosome in CTLs involves at least two distinct steps, with Lck signaling required for the centrosome to dock at the plasma membrane.

Original languageEnglish
Pages (from-to)663-674
Number of pages12
JournalJournal of Cell Biology
Volume192
Issue number4
DOIs
Publication statusPublished - 21 Feb 2011

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