Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study

Lynne I. McGuire; Anna Poleggi; Ilaria  Poggiolini; Silvia Suardi; Katarina  Grznarova; Song  Shi; Bart de Vil; Shannon  Sarros; Katsuya Satoh; Keding  Cheng; Maria  Cramm; Graham  Fairfoul; Matthias Schmitz; Inga Zerr; Patrick  Cras; Michele Equestre; Fabrizio Tagliavini; Ryuichiro  Atarashi; David  Knox; Steven Collins; Stéphane Haik; Piero Parchi; Maurizio Pocchiari; Alison Green

doi:10.1002/ana.24679

Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study

Lynne I. McGuire, Anna Poleggi, Ilaria Poggiolini , Silvia Suardi, Katarina Grznarova , Song Shi , Bart de Vil , Shannon Sarros , Katsuya Satoh, Keding Cheng , Maria Cramm , Graham Fairfoul , Matthias Schmitz, Inga Zerr, Patrick Cras , Michele Equestre, Fabrizio Tagliavini, Ryuichiro Atarashi , David Knox , Steven CollinsStéphane Haik, Piero Parchi, Maurizio Pocchiari, Alison Green

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Real‐time quaking‐induced conversion (RT‐QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt–Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT‐QuIC is a suitably reliable and robust technique for clinical practice.

Original language	English
Pages (from-to)	160-165
Number of pages	6
Journal	Annals of Neurology
Volume	80
Issue number	1
Early online date	30 Apr 2016
DOIs	https://doi.org/10.1002/ana.24679
Publication status	Published - 31 Jul 2016

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10.1002/ana.24679Licence: Creative Commons: Attribution (CC-BY)

McGuire_et_al-2016-Annals_of_NeurologyFinal published version, 83.1 KBLicence: Creative Commons: Attribution (CC-BY)

National CJD Research and Surveillance Unit
Knight, R., Green, A., Head, M., Molesworth, A., Smith, C. & Will, B.
UK central government bodies/local authorities, health and hospital authorities
1/07/14 → 31/03/17
Project: Research
Development & Evaluation of a Disease-Specific Diagnostics Test for Creutzfeldt-Jacob Disease Based on the Detection of PRPsc in the Cerebrospinal Fluid using Real-Time QUIC analysis
Green, A., Knight, R. & Will, B.
UK central government bodies/local authorities, health and hospital authorities
1/06/12 → 31/05/13
Project: Research
Optimisation, Harmonisation & Standardisation of Real-Time QulC analysis of CSF in the Diagnosis of Sporadic Creutzfeldt-Jakob Disease
McGuire, L. & Green, A.
MRC
2/04/12 → 31/12/15
Project: Research

Alison Green
- Centre for Clinical Brain Sciences
- Deanery of Clinical Sciences - Reader
Person: Academic: Research Active

Cite this

McGuire, L. I., Poleggi, A., Poggiolini , I., Suardi, S., Grznarova , K., Shi , S., de Vil , B., Sarros , S., Satoh, K., Cheng , K., Cramm , M., Fairfoul , G., Schmitz, M., Zerr, I., Cras , P., Equestre, M., Tagliavini, F., Atarashi , R., Knox , D., ... Green, A. (2016). Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study. Annals of Neurology, 80(1), 160-165. https://doi.org/10.1002/ana.24679

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title = "Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study",

abstract = "Real‐time quaking‐induced conversion (RT‐QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt–Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT‐QuIC is a suitably reliable and robust technique for clinical practice.",

author = "McGuire, {Lynne I.} and Anna Poleggi and Ilaria Poggiolini and Silvia Suardi and Katarina Grznarova and Song Shi and {de Vil}, Bart and Shannon Sarros and Katsuya Satoh and Keding Cheng and Maria Cramm and Graham Fairfoul and Matthias Schmitz and Inga Zerr and Patrick Cras and Michele Equestre and Fabrizio Tagliavini and Ryuichiro Atarashi and David Knox and Steven Collins and St{\'e}phane Haik and Piero Parchi and Maurizio Pocchiari and Alison Green",

year = "2016",

month = jul,

day = "31",

doi = "10.1002/ana.24679",

language = "English",

volume = "80",

pages = "160--165",

journal = "Annals of Neurology",

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McGuire, LI, Poleggi, A, Poggiolini , I, Suardi, S, Grznarova , K, Shi , S, de Vil , B, Sarros , S, Satoh, K, Cheng , K, Cramm , M, Fairfoul , G, Schmitz, M, Zerr, I, Cras , P, Equestre, M, Tagliavini, F, Atarashi , R, Knox , D, Collins, S, Haik, S, Parchi, P, Pocchiari, M & Green, A 2016, 'Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study', Annals of Neurology, vol. 80, no. 1, pp. 160-165. https://doi.org/10.1002/ana.24679

TY - JOUR

T1 - Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease

T2 - An international study

AU - McGuire, Lynne I.

AU - Poleggi, Anna

AU - Poggiolini , Ilaria

AU - Suardi, Silvia

AU - Grznarova , Katarina

AU - Shi , Song

AU - de Vil , Bart

AU - Sarros , Shannon

AU - Satoh, Katsuya

AU - Cheng , Keding

AU - Cramm , Maria

AU - Fairfoul , Graham

AU - Schmitz, Matthias

AU - Zerr, Inga

AU - Cras , Patrick

AU - Equestre, Michele

AU - Tagliavini, Fabrizio

AU - Atarashi , Ryuichiro

AU - Knox , David

AU - Collins, Steven

AU - Haik, Stéphane

AU - Parchi, Piero

AU - Pocchiari, Maurizio

AU - Green, Alison

PY - 2016/7/31

Y1 - 2016/7/31

N2 - Real‐time quaking‐induced conversion (RT‐QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt–Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT‐QuIC is a suitably reliable and robust technique for clinical practice.

AB - Real‐time quaking‐induced conversion (RT‐QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt–Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT‐QuIC is a suitably reliable and robust technique for clinical practice.

U2 - 10.1002/ana.24679

DO - 10.1002/ana.24679

M3 - Article

SN - 0364-5134

VL - 80

SP - 160

EP - 165

JO - Annals of Neurology

JF - Annals of Neurology

IS - 1

ER -

Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt–jakob disease: An international study

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Projects

National CJD Research and Surveillance Unit

Development & Evaluation of a Disease-Specific Diagnostics Test for Creutzfeldt-Jacob Disease Based on the Detection of PRPsc in the Cerebrospinal Fluid using Real-Time QUIC analysis

Optimisation, Harmonisation & Standardisation of Real-Time QulC analysis of CSF in the Diagnosis of Sporadic Creutzfeldt-Jakob Disease

Profiles

Alison Green

Cite this