Changes in chromatin structure during processing of wax-embedded tissue sections

Elizabeth Kerr, Tomoharu Kiyuna, Shelagh Boyle, Akira Saito, Jeremy Thomas, Wendy Bickmore

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The use of immunofluorescence (IF) and fluorescence in situ hybridisation (FISH) underpins much of our understanding of how chromatin is organised in the nucleus. However, there has only recently been an appreciation that these types of study need to move away from cells grown in culture and towards an investigation of nuclear organisation in cells in situ in their normal tissue architecture. Such analyses, however, especially of archival clinical samples, often requires use of formalin-fixed paraffin wax-embedded tissue sections which need addition steps of processing prior to IF or FISH. Here we quantify the changes in nuclear and chromatin structure that may be caused by these additional processing steps. Treatments, especially the microwaving to reverse fixation, do significantly alter nuclear architecture and chromatin texture, and these must be considered when inferring the original organisation of the nucleus from data collected from wax-embedded tissue sections.

Original languageEnglish
Pages (from-to)677-688
Number of pages12
JournalChromosome Research
Issue number6
Publication statusPublished - Sept 2010


Dive into the research topics of 'Changes in chromatin structure during processing of wax-embedded tissue sections'. Together they form a unique fingerprint.

Cite this