Changes in gastrointestinal morphology associated with obstructive jaundice

C H Stuart Cameron, C D Gannon, C Pope, T Diamond, B J Rowlands, Rowan W Parks

Research output: Contribution to journalArticlepeer-review

Abstract

Bacterial translocation has been consistently demonstrated in experimental models of obstructive jaundice. An important factor which promotes this phenomenon is physical injury of the intestinal mucosa. Some previous studies have presented suggestive evidence of this, following bile duct ligation. The aims of this study were to analyse objectively intestinal mucosal morphometric characteristics, to examine for evidence of bacterial translocation, and to assess enterocytes for ultrastructural abnormalities. Adult female Wistar rats were assigned to one of three groups: control (n=8), bile duct ligation (BDL; n=11), or sham operation (n=10). One week later, portal blood, mesenteric lymph nodes, liver, and spleen were harvested and cultured aerobically and anaerobically for evidence of bacterial translocation. Segments of jejunum, ileum, caecum, and large bowel were examined histologically, using light microscopy and morphometrically, using an image analysis system. Electron microscopy was performed on regions of the gastrointestinal tract where significant morphometric alterations had been identified. Significant bacterial translocation was identified following BDL (63. 6% BDL vs. 0% sham vs. 0% control, p
Original languageEnglish
Pages (from-to)526-32
Number of pages7
JournalThe Journal of Pathology
Volume192
Issue number4
DOIs
Publication statusPublished - 2000

Keywords

  • Animals
  • Bacterial Translocation
  • Cholestasis, Extrahepatic
  • Escherichia coli
  • Female
  • Ileum
  • Intestinal Mucosa
  • Male
  • Microscopy, Electron
  • Rats
  • Rats, Wistar

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