Changes in ovine conceptus and endometrial function following asynchronous embryo transfer or administration of progesterone

Cheryl Ashworth, Fuller W Bazer

Research output: Contribution to journalArticlepeer-review

Abstract

The secretory protein profile from conceptuses collected from naturally mated ewes on Days 10, 12, 14, and 16 was characterized by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and fluorography. The presence of the anti-luteolysin ovine trophoblast protein-1 (oTP-1) in culture medium from Day 10 conceptuses was confirmed by fluorography, Western blotting, and radioimmunoassay (RIA). On each of the days studied, oTP-1 was the dominant secretory protein, and was secreted in increasing quantities as pregnancy progressed. In a second experiment, Day 6 embryos were transferred to either Day 6 (SR) or Day 4 (AR) recipients. Three mated ewes (P) received daily injections of 50 mg progesterone on Days 4-9. Controls consisted of 2 groups of pregnant ewes (D8 and D10). Conceptuses and ipsilateral endometrium were collected 4 days following transfer of conceptuses to SR and AR ewes, on Day 10 in P and D10 ewes, and on Day 8 in D8 ewes. Conceptus volume was estimated upon recovery from the uterus. Tissues were cultured with 35S-methionine, and the medium was analyzed for total and trichloroacetic acid-precipitable radiolabeled proteins. Levels of specific endometrial secretory proteins were determined after protein separation by 2D-PAGE and estimation of the radioactivity associated with discrete radiolabeled proteins on fluorographs. The concentration of oTP-1 in conceptus culture medium was estimated by RIA. Thirty endometrial proteins were investigated. All 30 proteins were present in endometrial cultures from SR, AR, D10, and P ewes, but 13 proteins were absent from D8 ewes.Levels of three proteins were higher in AR compared to D8 (p<0.05).(p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS).
Original languageEnglish
Pages (from-to)425-33
Number of pages9
JournalBiology of Reproduction
Volume40
Issue number2
Publication statusPublished - Feb 1989

Keywords

  • Animals
  • Embryo Transfer
  • Embryo, Mammalian/physiology
  • Embryonic and Fetal Development/drug effects
  • Endometrium/drug effects
  • Endometrium/physiopathology
  • Endometrium/secretion
  • Female
  • Gestational Age
  • Interferon Type I
  • Pregnancy
  • Pregnancy Proteins/analysis
  • Pregnancy Proteins/secretion
  • Progesterone/pharmacology
  • Sheep

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