CHANGES IN XYLOGLUCAN ENDOTRANSGLYCOSYLASE (XET) ACTIVITY DURING HORMONE-INDUCED GROWTH IN LETTUCE AND CUCUMBER HYPOCOTYLS AND SPINACH CELL-SUSPENSION CULTURES

I POTTER, Stephen Fry

Research output: Contribution to journalArticlepeer-review

Abstract

The promotion of stem elongation in dwarf pea plants by GA(3) has been correlated with an increase in extractable xyloglucan endotransglycosylase (XET) activity (Potter and Fry, 1993). Doses of auxin that caused either elongation or lateral swelling in pea stems have not been found to increase XET activity (Fry et al., 1992). We therefore explored the generality of the association between GA(3) action and XET activity by testing three other bioassay systems.

A similar correlation was found in the hypocotyls of intact lettuce seedlings, where GA(3) strongly promoted elongation and doubled the extractable XET activity per unit fresh weight.

In the hypocotyls of intact cucumber seedlings, GA(3) evoked a prolonged promotion of elongation for at least 96 h; this was correlated with only a small increase in XET activity per unit fresh weight of tissue. IAA promoted elongation for only 48 h, but this effect was correlated with a larger increase in XET activity per unit fresh weight than that due to GA(3).

In cell suspension cultures of spinach, much of the XET activity was present in solution in the culture medium. GA(3) had little effect on this fraction for the first 9 d, but thereafter the hormone suppressed a sudden burst in soluble extracellular XET activity that occurred in the untreated controls. A further proportion of the XET activity was ionically wall-bound; this fraction was enhanced by GA(3) in the early phase of the growth cycle of the culture and inhibited in later phases. Pre-adaptation of the culture by growth for 6 years in the presence of 10(-7) M GA(3) intensified the response of the cells to re-addition of GA(3).

We conclude that there is no simple or unique relationship between total extractable XET activity and GA(3) action. Nevertheless, we have extended the evidence that XET is often subject to the effects of GA(3) in systems where this hormone influences growth.

Original languageEnglish
Pages (from-to)1703-1710
Number of pages8
JournalJournal of Experimental Botany
Volume45
Issue number280
Publication statusPublished - Nov 1994

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