Chapter 13: The Application of the Comet Assay in Aquatic Environments

G. Frenzilli, T. P. Bean, B. P. Lyons

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract / Description of output

This chapter considers the potential of the Comet assay [or single-cell gel electrophoresis (SCGE)] for evaluating the environmental impact of genotoxins in aquatic environments. It focuses on in vivo and in situ studies that have been carried out using various marine and freshwater sentinel species. A large number of the studies reviewed report that the Comet assay is more sensitive when compared with other biomarkers commonly used in genetic ecotoxicology, such as sister chromatid exchanges or the micronucleus test. The Comet assay is widely influenced by laboratory procedures, suggesting that standard protocols are required for both vertebrate and invertebrate species and tissues. However, there is still a wide variety of differing Comet procedures evident in the literature reviewed, making comparison between published results often very difficult. Standardization and inter-laboratory calibration of the Comet assay as applied to aquatic species will be required if the Comet assay is to be used routinely by national bodies charged with monitoring water quality.

Original languageEnglish
Title of host publicationThe Comet Assay in Toxicology, 2nd Edition
EditorsDiana Anderson, Alok Dhawan
PublisherRoyal Society of Chemistry
Pages354-368
Number of pages15
Edition30
DOIs
Publication statusPublished - 1 Jan 2017

Publication series

NameIssues in Toxicology
Number30
Volume2017-January
ISSN (Print)1757-7179
ISSN (Electronic)1757-7187

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