Chapter 7. Nonsense-mediated mRNA decay in Caenorhabditis elegans

Dasa Longman, Pablo Arrisi, Iain L Johnstone, Javier F Cáceres

Research output: Contribution to journalArticlepeer-review

Abstract

The nonsense-mediated mRNA decay (NMD) pathway is a surveillance mechanism that targets the degradation of mRNAs harboring premature termination codons (PTCs). Two key aspects of NMD are the definition of a PTC codon and the identification of the molecular machinery dedicated to this mechanism. This chapter describes the development of transgenic reporters as well as the use of genome-wide RNAi and genetic screens to identify novel components of the NMD pathway in the nematode Caenorhabditis elegans.
Original languageEnglish
Pages (from-to)149-64
Number of pages16
JournalMethods in enzymology
Volume449
DOIs
Publication statusPublished - 2008

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