Abstract / Description of output
The nonsense-mediated mRNA decay (NMD) pathway is a surveillance mechanism that targets the degradation of mRNAs harboring premature termination codons (PTCs). Two key aspects of NMD are the definition of a PTC codon and the identification of the molecular machinery dedicated to this mechanism. This chapter describes the development of transgenic reporters as well as the use of genome-wide RNAi and genetic screens to identify novel components of the NMD pathway in the nematode Caenorhabditis elegans.
Original language | English |
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Pages (from-to) | 149-64 |
Number of pages | 16 |
Journal | Methods in enzymology |
Volume | 449 |
DOIs | |
Publication status | Published - 2008 |