Characterisation and expression analysis of the chicken interleukin-7 receptor alpha chain

D.A. van Haarlem, P.J. van Kooten, L. Rothwell, P. Kaiser, Lonneke Vervelde

Research output: Contribution to journalArticlepeer-review

Abstract

Interleukin-7 (IL-7) is a central regulator of T cell survival and homeostasis and its expression is indicative for naive and memory T cells. We cloned chicken IL-7Ralpha (CHIL-7Ralpha) and determined its expression profile in chicken lymphocyte subpopulations. The predicted protein sequence contained 460 amino acids. The extracellular domain exhibited features typical of a type I cytokine receptor; a fibronectin type III domain and the GXWSXWS motif were conserved. ChIL-7Ralpha mRNA is highly expressed in lymphoid organs and in CD4+, CD8alpha+ and CD8beta+ cells. A monoclonal antibody was generated and expression of the protein investigated. ChIL-7Ralpha was expressed on CD4+ and CD8alpha+, but not CD8beta+, T cells, in contrast to the high mRNA expression levels in all of these cells. Upon polyclonal stimulation with ConA, IL-7Ralpha was rapidly down-regulated on T cells, suggesting that in the chicken expression of this receptor might also be correlated to the T cell activation status.
Original languageEnglish
Pages (from-to)1018-1026
Number of pages9
JournalDevelopmental and Comparative Immunology
Volume33
Issue number9
DOIs
Publication statusPublished - 2009

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chickens/immunology
  • Concanavalin A/pharmacology
  • Down-Regulation/drug effects
  • Down-Regulation/immunology
  • Lymphocyte Activation/drug effects
  • Molecular Sequence Data
  • Receptors, Interleukin-7/biosynthesis
  • Receptors, Interleukin-7/immunology
  • Sequence Alignment
  • T-Lymphocyte Subsets/drug effects
  • T-Lymphocyte Subsets/immunology

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