Characterisation of a monoclonal antibody recognising the CD3epsilon chain of the bovine T cell receptor complex

Niall MacHugh, JK Mburu, MJ Hamilton, W.C. Davis

Research output: Contribution to journalArticlepeer-review

Abstract

We describe the characterisation of a monoclonal antibody (mAb), designated MM1A, which reacts with an antigen molecule on the surface of bovine alphabeta and gammadeltaTcR+ T cells. The mAb immunoprecipitated a series of polypeptides of 21 kDa, 22 kDa, 32 kDa, 36 kDa and 44 kDa which is consistent with it recognising the TcR/CD3 complex. COS cells, transfected with a cDNA encoding the bovine CD3epsilon chain, reacted with mAb MM1A indicating that the epitope recognised is on the epsilon chain of the complex and confirming that the mAb recognised bovine CD3.
Original languageEnglish
Pages (from-to)25-35
Number of pages11
JournalVeterinary Immunology and Immunopathology
Volume61
Issue number1
Publication statusPublished - 16 Feb 1998

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Antigens, CD3
  • COS Cells
  • Cattle/immunology
  • Cell Line, Transformed
  • Electrophoresis, Polyacrylamide Gel
  • Epitopes, T-Lymphocyte
  • Flow Cytometry/veterinary
  • Immunoenzyme Techniques
  • Lymph Nodes/chemistry
  • Mice
  • Molecular Weight
  • Peptides/analysis
  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, Antigen, T-Cell, gamma-delta
  • Transfection

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