Characterisation of a monoclonal antibody recognising the CD3epsilon chain of the bovine T cell receptor complex

Niall MacHugh; JK Mburu; MJ Hamilton; W.C. Davis

Characterisation of a monoclonal antibody recognising the CD3epsilon chain of the bovine T cell receptor complex

Niall MacHugh, JK Mburu, MJ Hamilton, W.C. Davis

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

We describe the characterisation of a monoclonal antibody (mAb), designated MM1A, which reacts with an antigen molecule on the surface of bovine alphabeta and gammadeltaTcR+ T cells. The mAb immunoprecipitated a series of polypeptides of 21 kDa, 22 kDa, 32 kDa, 36 kDa and 44 kDa which is consistent with it recognising the TcR/CD3 complex. COS cells, transfected with a cDNA encoding the bovine CD3epsilon chain, reacted with mAb MM1A indicating that the epitope recognised is on the epsilon chain of the complex and confirming that the mAb recognised bovine CD3.

Original language	English
Pages (from-to)	25-35
Number of pages	11
Journal	Veterinary Immunology and Immunopathology
Volume	61
Issue number	1
Publication status	Published - 16 Feb 1998

Keywords

Animals
Antibodies, Monoclonal
Antigens, CD3
COS Cells
Cattle/immunology
Cell Line, Transformed
Electrophoresis, Polyacrylamide Gel
Epitopes, T-Lymphocyte
Flow Cytometry/veterinary
Immunoenzyme Techniques
Lymph Nodes/chemistry
Mice
Molecular Weight
Peptides/analysis
Receptors, Antigen, T-Cell, alpha-beta
Receptors, Antigen, T-Cell, gamma-delta
Transfection

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Cite this

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title = "Characterisation of a monoclonal antibody recognising the CD3epsilon chain of the bovine T cell receptor complex",

abstract = "We describe the characterisation of a monoclonal antibody (mAb), designated MM1A, which reacts with an antigen molecule on the surface of bovine alphabeta and gammadeltaTcR+ T cells. The mAb immunoprecipitated a series of polypeptides of 21 kDa, 22 kDa, 32 kDa, 36 kDa and 44 kDa which is consistent with it recognising the TcR/CD3 complex. COS cells, transfected with a cDNA encoding the bovine CD3epsilon chain, reacted with mAb MM1A indicating that the epitope recognised is on the epsilon chain of the complex and confirming that the mAb recognised bovine CD3.",

keywords = "Animals, Antibodies, Monoclonal, Antigens, CD3, COS Cells, Cattle/immunology, Cell Line, Transformed, Electrophoresis, Polyacrylamide Gel, Epitopes, T-Lymphocyte, Flow Cytometry/veterinary, Immunoenzyme Techniques, Lymph Nodes/chemistry, Mice, Molecular Weight, Peptides/analysis, Receptors, Antigen, T-Cell, alpha-beta, Receptors, Antigen, T-Cell, gamma-delta, Transfection",

author = "Niall MacHugh and JK Mburu and MJ Hamilton and W.C. Davis",

year = "1998",

month = feb,

day = "16",

language = "English",

volume = "61",

pages = "25--35",

journal = "Veterinary Immunology and Immunopathology",

issn = "0165-2427",

publisher = "Elsevier",

number = "1",

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TY - JOUR

T1 - Characterisation of a monoclonal antibody recognising the CD3epsilon chain of the bovine T cell receptor complex

AU - MacHugh, Niall

AU - Mburu, JK

AU - Hamilton, MJ

AU - Davis, W.C.

PY - 1998/2/16

Y1 - 1998/2/16

N2 - We describe the characterisation of a monoclonal antibody (mAb), designated MM1A, which reacts with an antigen molecule on the surface of bovine alphabeta and gammadeltaTcR+ T cells. The mAb immunoprecipitated a series of polypeptides of 21 kDa, 22 kDa, 32 kDa, 36 kDa and 44 kDa which is consistent with it recognising the TcR/CD3 complex. COS cells, transfected with a cDNA encoding the bovine CD3epsilon chain, reacted with mAb MM1A indicating that the epitope recognised is on the epsilon chain of the complex and confirming that the mAb recognised bovine CD3.

AB - We describe the characterisation of a monoclonal antibody (mAb), designated MM1A, which reacts with an antigen molecule on the surface of bovine alphabeta and gammadeltaTcR+ T cells. The mAb immunoprecipitated a series of polypeptides of 21 kDa, 22 kDa, 32 kDa, 36 kDa and 44 kDa which is consistent with it recognising the TcR/CD3 complex. COS cells, transfected with a cDNA encoding the bovine CD3epsilon chain, reacted with mAb MM1A indicating that the epitope recognised is on the epsilon chain of the complex and confirming that the mAb recognised bovine CD3.

KW - Animals

KW - Antibodies, Monoclonal

KW - Antigens, CD3

KW - COS Cells

KW - Cattle/immunology

KW - Cell Line, Transformed

KW - Electrophoresis, Polyacrylamide Gel

KW - Epitopes, T-Lymphocyte

KW - Flow Cytometry/veterinary

KW - Immunoenzyme Techniques

KW - Lymph Nodes/chemistry

KW - Mice

KW - Molecular Weight

KW - Peptides/analysis

KW - Receptors, Antigen, T-Cell, alpha-beta

KW - Receptors, Antigen, T-Cell, gamma-delta

KW - Transfection

M3 - Article

VL - 61

SP - 25

EP - 35

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 1

ER -

Characterisation of a monoclonal antibody recognising the CD3epsilon chain of the bovine T cell receptor complex

Abstract

Keywords

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