Characterisation of a nucleo-adhesome

Adam Byron*, Billie Griffith, Ana Herrero Mier, Alexander E.P. Loftus, Emma S. Koeleman, Linda Kogerman, John C Dawson, Niamh McGivern, Jayne Culley, Graeme R Grimes, Bryan Serrels, Alexander von Kriegsheim, Valerie G Brunton, Margaret C Frame

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

In addition to central functions in cell adhesion signalling, integrin-associated proteins have wider roles at sites distal to adhesion receptors. In experimentally defined adhesomes, we noticed that there is clear enrichment of proteins that localise to the nucleus, and conversely, we now report that nuclear proteomes contain a class of adhesome components that localise to the nucleus. We here
define a nucleo-adhesome, providing experimental evidence for a remarkable scale of nuclear localisation of adhesion proteins, establishing a framework for interrogating nuclear adhesion protein functions. In adding to nuclear FAK’s known roles in regulating transcription, we now show that nuclear FAK regulates expression of many adhesion-related proteins that localise to the nucleus and that nuclear FAK binds to the adhesome component and nuclear protein Hic-5. FAK and Hic-5 work together in the nucleus, co-regulating a subset of genes transcriptionally. We demonstrate the principle that there are subcomplexes of nuclear adhesion proteins that cooperate to control transcription
Original languageEnglish
JournalNature Communications
Early online date1 Jun 2022
DOIs
Publication statusE-pub ahead of print - 1 Jun 2022

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