Characterisation of cyclin D1 down-regulation in coronavirus infected cells

S M Harrison, B. K. Dove, L Rothwell, Pete Kaiser, I Tarpey, G. Brooks, J.A. Hiscox

Research output: Contribution to journalArticlepeer-review

Abstract

The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells independently of the cell-cycle stage at the time of infection. Confocal microscopy revealed that cyclin D1 decreased in IBV-infected cells as infection progressed and inhibition studies indicated that a population of cyclin D1 could be targeted for degradation by a virus mediated pathway. In contrast to the SARS-coronavirus, IBV nucleocapsid protein did not interact with cyclin D1.
Original languageEnglish
Pages (from-to)1275-1286
Number of pages12
JournalFEBS Letters
Volume581
Issue number7
DOIs
Publication statusPublished - 2007

Keywords

  • Animals
  • Blotting, Western
  • Cercopithecus aethiops
  • Coronavirus
  • Cyclin D1
  • Down-Regulation
  • Infectious bronchitis virus
  • RNA, Messenger
  • RNA, Viral/analysis
  • RNA, Viral/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Vero Cells

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