Characterisation of gamma-glutamylcysteine synthetase-heavy subunit promoter: a critical role for AP-1

Irfan Rahman, Christopher A D Smith, Frank Antonicelli, W Macnee

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The 5'-flanking region of human gamma-glutamylcysteine synthetase-heavy subunit (gamma-GCS-HS) was characterised by creating a series of chloramphenicol acetyl transferase (CAT) reporter deletion constructs. Analysis of various deleted CAT constructs revealed that a putative AP-1 consensus sequence is required to direct the constitutive and oxidant-mediated promoter activity. Gel mobility shift and mutation analysis of the sequence (-269 to -263 bp), showed binding of AP-1 is involved in the oxidant-mediated regulation of gamma-GCS-HS promoter activity.
Original languageEnglish
Pages (from-to)129-33
Number of pages5
JournalFEBS Letters
Volume427
Issue number1
DOIs
Publication statusPublished - 1 May 1998

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