Characterisation of hyaluronic acid and chondroitin/dermatan sulfate from the lumpsucker fish, C. lumpus

Charalampos G. Panagos; Derek Thomson; Claire Moss; Charles D. Bavington; Halldor G. Olafsson; Dusan Uhrin

doi:10.1016/j.carbpol.2014.01.090

Characterisation of hyaluronic acid and chondroitin/dermatan sulfate from the lumpsucker fish, C. lumpus

Charalampos G. Panagos, Derek Thomson, Claire Moss, Charles D. Bavington, Halldor G. Olafsson, Dusan Uhrin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The lumpsucker, Cyclopterus lumpus, a cottoid teleost fish found in the cold waters of the North Atlantic, and North Pacific, was identified as a possible source of GAGs. The GAGs present in the C. lumpus dorsal hump and body wall tissue were isolated and purified. Two fractions were analysed by NMR and their GAG structures determined as hyaluronic acid and CS/DS chains. The latter fraction contained GIcA (65% of the total uronic acids) and IdoA (the remaining 35%). All uronic acid residues were unsulfated, whilst 86% of the GalNAc was 4-sulfated and 14% was 6-sulfated. The presence of GlcA-GalNAc4S, IdoA-GalNAc4S and GlcA-GalNAc6S disaccharide fragments was confirmed. The isolated GAGs obtained from each tissue were biochemically characterised. The lumpsucker offers a high yield source of GAGs, which compares favourably with other sources such as shark cartilage. (c) 2014 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	25-33
Journal	Carbohydrate polymers
Volume	106
Early online date	5 Feb 2014
DOIs	https://doi.org/10.1016/j.carbpol.2014.01.090
Publication status	Published - 15 Jun 2014

Keywords / Materials (for Non-textual outputs)

Cyclopterus lumpus
Chondroitin sulfate
Dermatan sulfate
Hyaluronic acid
Glycosaminoglycan
NMR
MASS-SPECTROMETRIC ANALYSIS
DERMATAN SULFATE
SEA-CUCUMBER
STRUCTURAL-CHARACTERIZATION
ANTIINFLAMMATORY ACTIVITY
ANTICOAGULANT ACTIVITIES
MARINE-INVERTEBRATES
PROTEIN INTERACTIONS
CONNECTIVE-TISSUE
HEPARAN-SULFATE

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10.1016/j.carbpol.2014.01.090

http://www.sciencedirect.com/science/article/pii/S014486171400099X

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@article{ec70a67b18be429fb91afbd764fbf187,

title = "Characterisation of hyaluronic acid and chondroitin/dermatan sulfate from the lumpsucker fish, C. lumpus",

abstract = "The lumpsucker, Cyclopterus lumpus, a cottoid teleost fish found in the cold waters of the North Atlantic, and North Pacific, was identified as a possible source of GAGs. The GAGs present in the C. lumpus dorsal hump and body wall tissue were isolated and purified. Two fractions were analysed by NMR and their GAG structures determined as hyaluronic acid and CS/DS chains. The latter fraction contained GIcA (65\% of the total uronic acids) and IdoA (the remaining 35\%). All uronic acid residues were unsulfated, whilst 86\% of the GalNAc was 4-sulfated and 14\% was 6-sulfated. The presence of GlcA-GalNAc4S, IdoA-GalNAc4S and GlcA-GalNAc6S disaccharide fragments was confirmed. The isolated GAGs obtained from each tissue were biochemically characterised. The lumpsucker offers a high yield source of GAGs, which compares favourably with other sources such as shark cartilage. (c) 2014 Elsevier Ltd. All rights reserved.",

keywords = "Cyclopterus lumpus, Chondroitin sulfate, Dermatan sulfate, Hyaluronic acid, Glycosaminoglycan, NMR, MASS-SPECTROMETRIC ANALYSIS, DERMATAN SULFATE, SEA-CUCUMBER, STRUCTURAL-CHARACTERIZATION, ANTIINFLAMMATORY ACTIVITY, ANTICOAGULANT ACTIVITIES, MARINE-INVERTEBRATES, PROTEIN INTERACTIONS, CONNECTIVE-TISSUE, HEPARAN-SULFATE",

author = "Panagos, \{Charalampos G.\} and Derek Thomson and Claire Moss and Bavington, \{Charles D.\} and Olafsson, \{Halldor G.\} and Dusan Uhrin",

year = "2014",

month = jun,

day = "15",

doi = "10.1016/j.carbpol.2014.01.090",

language = "English",

volume = "106",

pages = "25--33",

journal = "Carbohydrate polymers",

issn = "0144-8617",

publisher = "Elsevier",

}

TY - JOUR

T1 - Characterisation of hyaluronic acid and chondroitin/dermatan sulfate from the lumpsucker fish, C. lumpus

AU - Panagos, Charalampos G.

AU - Thomson, Derek

AU - Moss, Claire

AU - Bavington, Charles D.

AU - Olafsson, Halldor G.

AU - Uhrin, Dusan

PY - 2014/6/15

Y1 - 2014/6/15

N2 - The lumpsucker, Cyclopterus lumpus, a cottoid teleost fish found in the cold waters of the North Atlantic, and North Pacific, was identified as a possible source of GAGs. The GAGs present in the C. lumpus dorsal hump and body wall tissue were isolated and purified. Two fractions were analysed by NMR and their GAG structures determined as hyaluronic acid and CS/DS chains. The latter fraction contained GIcA (65% of the total uronic acids) and IdoA (the remaining 35%). All uronic acid residues were unsulfated, whilst 86% of the GalNAc was 4-sulfated and 14% was 6-sulfated. The presence of GlcA-GalNAc4S, IdoA-GalNAc4S and GlcA-GalNAc6S disaccharide fragments was confirmed. The isolated GAGs obtained from each tissue were biochemically characterised. The lumpsucker offers a high yield source of GAGs, which compares favourably with other sources such as shark cartilage. (c) 2014 Elsevier Ltd. All rights reserved.

AB - The lumpsucker, Cyclopterus lumpus, a cottoid teleost fish found in the cold waters of the North Atlantic, and North Pacific, was identified as a possible source of GAGs. The GAGs present in the C. lumpus dorsal hump and body wall tissue were isolated and purified. Two fractions were analysed by NMR and their GAG structures determined as hyaluronic acid and CS/DS chains. The latter fraction contained GIcA (65% of the total uronic acids) and IdoA (the remaining 35%). All uronic acid residues were unsulfated, whilst 86% of the GalNAc was 4-sulfated and 14% was 6-sulfated. The presence of GlcA-GalNAc4S, IdoA-GalNAc4S and GlcA-GalNAc6S disaccharide fragments was confirmed. The isolated GAGs obtained from each tissue were biochemically characterised. The lumpsucker offers a high yield source of GAGs, which compares favourably with other sources such as shark cartilage. (c) 2014 Elsevier Ltd. All rights reserved.

KW - Cyclopterus lumpus

KW - Chondroitin sulfate

KW - Dermatan sulfate

KW - Hyaluronic acid

KW - Glycosaminoglycan

KW - NMR

KW - MASS-SPECTROMETRIC ANALYSIS

KW - DERMATAN SULFATE

KW - SEA-CUCUMBER

KW - STRUCTURAL-CHARACTERIZATION

KW - ANTIINFLAMMATORY ACTIVITY

KW - ANTICOAGULANT ACTIVITIES

KW - MARINE-INVERTEBRATES

KW - PROTEIN INTERACTIONS

KW - CONNECTIVE-TISSUE

KW - HEPARAN-SULFATE

U2 - 10.1016/j.carbpol.2014.01.090

DO - 10.1016/j.carbpol.2014.01.090

M3 - Article

SN - 0144-8617

VL - 106

SP - 25

EP - 33

JO - Carbohydrate polymers

JF - Carbohydrate polymers

ER -

Characterisation of hyaluronic acid and chondroitin/dermatan sulfate from the lumpsucker fish, C. lumpus

Abstract

Keywords / Materials (for Non-textual outputs)

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