Characterisation of microsatellite loci in silver carp and cross amplification in other cyprinid species

Almas Gheyas, M. Cairney, A.E. Gilmour, M.A. Sattar, T.K. Das, B.J. McAndrew, D.J. Penman, J.B. Taggart

Research output: Contribution to journalArticlepeer-review

Abstract

Captive populations of silver carp (Hypophthalmichthys molitrix), a major aquaculture species in Asia, would undoubtedly benefit from genetic monitoring and improvement programs. We report the isolation and preliminary characterization of 16 microsatellite loci derived from both conventional and microsatellite-enriched libraries. Inheritance studies confirmed the allelic nature of observed polymorphisms at all loci, while identifying null alleles at two loci. These loci, having varying degrees of polymorphism, should provide useful markers for applied genetic studies. A high degree of cross-amplification among 10 other cyprinid species suggests that these loci may have more widespread utility.
Original languageEnglish
Pages (from-to)656-659
JournalMolecular Ecology Notes
Volume6
DOIs
Publication statusPublished - 6 Jun 2006

Keywords

  • cross-species amplification
  • genetic markers
  • inheritance
  • microsatellite enrichment
  • silver carp

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