Characterisation of the Avascular Mesenchyme during Digit Outgrowth

Cameron Batho-Samblas; Jonathan Smith; Lois Keavey; Noah  Clancy; Lynn McTeir; Megan G Davey

doi:10.1016/j.ydbio.2025.04.005

Characterisation of the Avascular Mesenchyme during Digit Outgrowth

Cameron Batho-Samblas, Jonathan Smith, Lois Keavey, Noah Clancy, Lynn McTeir, Megan G Davey^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The avascular mesenchyme at the tip of the developing digit contributes to digit outgrowth and patterning, however, it has been poorly characterised. Using newly developed fate mapping approaches, tissue manipulation and single-cell mRNA sequencing data, we explore the transcriptional nature and developmental potential of this tissue. We find that the avascular mesenchyme is essential to normal segmental patterning of the digit and has a distinct transcriptional identity. In addition, we uncover an unexpected relationship between the unspecified tissue of the avascular mesenchyme and the committed phalanx forming region, which controls patterning, but not outgrowth of the digit. This multifaceted approach provides insights into the mechanics and genetic pathways that regulate digit outgrowth and patterning.

Original language	English
Pages (from-to)	99-110
Number of pages	12
Journal	Developmental Biology
Volume	523
Early online date	8 Apr 2025
DOIs	https://doi.org/10.1016/j.ydbio.2025.04.005
Publication status	E-pub ahead of print - 8 Apr 2025

Keywords / Materials (for Non-textual outputs)

digit
AVM
PFR
FGF8
brachydacyly
chicken
HMGA1
PRICKLE1

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10.1016/j.ydbio.2025.04.005

1-s2.0-S0012160625000983-mainFinal published version, 11.4 MBLicence: CC BY

1 Finished
3 Active

Genetic and Molecular Regulation of Beak Tip (Rhinotheca) Shape in Layer Hens
Schoenebeck, J. (Principal Investigator), Davey, M. (Co-investigator), McGrew, M. (Co-investigator) & Prendergast, J. (Co-investigator)
BBSRC
1/10/23 → 30/09/26
Project: Research
ISP 1 2023/28 Genes & Traits for Healthy Animals
Headon, D. (Principal Investigator)
1/04/23 → 31/03/28
Project: Research
Understanding the origins of animal phenotypes
Headon, D. (Principal Investigator)
1/04/23 → 31/03/28
Project: Research

Cite this

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title = "Characterisation of the Avascular Mesenchyme during Digit Outgrowth",

abstract = "The avascular mesenchyme at the tip of the developing digit contributes to digit outgrowth and patterning, however, it has been poorly characterised. Using newly developed fate mapping approaches, tissue manipulation and single-cell mRNA sequencing data, we explore the transcriptional nature and developmental potential of this tissue. We find that the avascular mesenchyme is essential to normal segmental patterning of the digit and has a distinct transcriptional identity. In addition, we uncover an unexpected relationship between the unspecified tissue of the avascular mesenchyme and the committed phalanx forming region, which controls patterning, but not outgrowth of the digit. This multifaceted approach provides insights into the mechanics and genetic pathways that regulate digit outgrowth and patterning.",

keywords = "digit, AVM, PFR, FGF8, brachydacyly, chicken, HMGA1, PRICKLE1",

author = "Cameron Batho-Samblas and Jonathan Smith and Lois Keavey and Noah Clancy and Lynn McTeir and Davey, {Megan G}",

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doi = "10.1016/j.ydbio.2025.04.005",

language = "English",

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journal = "Developmental Biology",

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T1 - Characterisation of the Avascular Mesenchyme during Digit Outgrowth

AU - Batho-Samblas, Cameron

AU - Smith, Jonathan

AU - Keavey, Lois

AU - Clancy, Noah

AU - McTeir, Lynn

AU - Davey, Megan G

PY - 2025/4/8

Y1 - 2025/4/8

N2 - The avascular mesenchyme at the tip of the developing digit contributes to digit outgrowth and patterning, however, it has been poorly characterised. Using newly developed fate mapping approaches, tissue manipulation and single-cell mRNA sequencing data, we explore the transcriptional nature and developmental potential of this tissue. We find that the avascular mesenchyme is essential to normal segmental patterning of the digit and has a distinct transcriptional identity. In addition, we uncover an unexpected relationship between the unspecified tissue of the avascular mesenchyme and the committed phalanx forming region, which controls patterning, but not outgrowth of the digit. This multifaceted approach provides insights into the mechanics and genetic pathways that regulate digit outgrowth and patterning.

AB - The avascular mesenchyme at the tip of the developing digit contributes to digit outgrowth and patterning, however, it has been poorly characterised. Using newly developed fate mapping approaches, tissue manipulation and single-cell mRNA sequencing data, we explore the transcriptional nature and developmental potential of this tissue. We find that the avascular mesenchyme is essential to normal segmental patterning of the digit and has a distinct transcriptional identity. In addition, we uncover an unexpected relationship between the unspecified tissue of the avascular mesenchyme and the committed phalanx forming region, which controls patterning, but not outgrowth of the digit. This multifaceted approach provides insights into the mechanics and genetic pathways that regulate digit outgrowth and patterning.

KW - digit

KW - AVM

KW - PFR

KW - FGF8

KW - brachydacyly

KW - chicken

KW - HMGA1

KW - PRICKLE1

U2 - 10.1016/j.ydbio.2025.04.005

DO - 10.1016/j.ydbio.2025.04.005

M3 - Article

C2 - 40210155

SN - 0012-1606

VL - 523

SP - 99

EP - 110

JO - Developmental Biology

JF - Developmental Biology

ER -

Characterisation of the Avascular Mesenchyme during Digit Outgrowth

Abstract

Keywords / Materials (for Non-textual outputs)

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Projects

Genetic and Molecular Regulation of Beak Tip (Rhinotheca) Shape in Layer Hens

ISP 1 2023/28 Genes & Traits for Healthy Animals

Understanding the origins of animal phenotypes

Cite this