TY - JOUR
T1 - Characterisation of twenty-one European badger (Meles meles) microsatellite loci facilitates the discrimination of second-order relatives
AU - Annavi, G.
AU - Dawson, D.A.
AU - Horsburgh, G.J.
AU - Greig, C.
AU - Dugdale, H.L.
AU - Newman, C.
AU - Macdonald, D.W.
AU - Burke, T.
PY - 2011/7/1
Y1 - 2011/7/1
N2 - The European badger (Meles meles) breeds plurally in lowland England and is important economically due to its link with bovine tuberculosis (Mycobacterium bovis) transmission. To understand disease transmission and facilitate effective management, it is vital to elucidate the social structure of badger groups. To improve parentage assignment and the discrimination of relatives, we isolated and characterised 21 polymorphic microsatellite loci in 24 individuals from Wytham Woods, Oxfordshire, UK. These 21 loci increased the discrimination power between fullsiblings and half-siblings from 71 to 88%, when added to the existing 31 loci. Similarly, the combined non-exclusion probability increased from 3.0 9 10 to 5.8 9 10 . Newly isolated Mel-592 (FR745854) was X-linked, based on the genotypes of 48 known-sex individuals and will enhance the genetic sex-typing of badgers.
AB - The European badger (Meles meles) breeds plurally in lowland England and is important economically due to its link with bovine tuberculosis (Mycobacterium bovis) transmission. To understand disease transmission and facilitate effective management, it is vital to elucidate the social structure of badger groups. To improve parentage assignment and the discrimination of relatives, we isolated and characterised 21 polymorphic microsatellite loci in 24 individuals from Wytham Woods, Oxfordshire, UK. These 21 loci increased the discrimination power between fullsiblings and half-siblings from 71 to 88%, when added to the existing 31 loci. Similarly, the combined non-exclusion probability increased from 3.0 9 10 to 5.8 9 10 . Newly isolated Mel-592 (FR745854) was X-linked, based on the genotypes of 48 known-sex individuals and will enhance the genetic sex-typing of badgers.
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84859835245&partnerID=8YFLogxK
U2 - 10.1007/s12686-011-9392-9
DO - 10.1007/s12686-011-9392-9
M3 - Article
AN - SCOPUS:84859835245
SN - 1877-7252
VL - 3
SP - 515
EP - 518
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 3
ER -