Characterising the structure of photosynthetic biofilms using fluid dynamic gauging

B. Salley, P. W. Gordon, A. J. McCormick, A. C. Fisher, D. I. Wilson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A new configuration of the fluid dynamic gauging technique for measuring soft layers on surfaces was used to monitor the growth of a cyanobacterium, Synechococcus sp. WH 5701, on stainless steel (SS), glass and an indium tin oxide (ITO) on a polyethylene terephthalate (PET) substratum. The biofilm thickness increased steadily over 4 weeks and exhibited noticeable changes in microstructure and strength. The biofilms all exhibited a two-layer structure, with a compact layer next to the substratum and a loose layer above. Biofilms on ITO or SS exhibited cohesive failure when removed by fluid shear whereas those on glass exhibited adhesive failure. The technique is able to elucidate various aspects of biofilm behaviour, as illustrated by the action of a biocide (NaOCl) on a mature biofilm.

Original languageEnglish
Pages (from-to)159-173
Number of pages15
JournalBiofouling
Volume28
Issue number2
DOIs
Publication statusPublished - 13 Feb 2012

Keywords

  • biofilms
  • fluid dynamic gauging
  • synechococcus sp WH 5701
  • surfaces
  • strength
  • thickness
  • bacterial adhesion
  • simutaneous-equations
  • CHLOROPHYLL-A
  • soft deposits
  • attachment
  • growth
  • energy
  • jet

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