Characterization and analysis of human arterial tissue secretome by 2-DE and nLC-MS/MS

Fernando de la Cuesta, Maria G Barderas, Enrique Calvo, Irene Zubiri, Aroa S Maroto, Juan Antonio Lopez, Fernando Vivanco, Gloria Alvarez-Llamas

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Early detection of cardiovascular diseases and knowledge of underlying mechanisms is essential. Tissue secretome studies resemble more closely to the in vivo situation, showing a much narrower protein concentrations dynamic range than plasma. In the present chapter, we detail the characterization and analysis of human arterial tissue secretome by two-dimensional electrophoresis (2-DE) and nano-liquid chromatography on-line coupled to mass spectrometry (nLC-MS/MS). General strategies shown here can be extended to other tissue secretome studies.

Original languageEnglish
Title of host publicationVascular Proteomics
Pages81-90
Number of pages10
Volume1000
DOIs
Publication statusPublished - 2013

Keywords

  • Arteries
  • Breast
  • Chromatography, Liquid
  • Coronary Vessels
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Nanotechnology
  • Peptide Fragments
  • Proteolysis
  • Proteomics
  • Tandem Mass Spectrometry

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