Abstract
Early detection of cardiovascular diseases and knowledge of underlying mechanisms is essential. Tissue secretome studies resemble more closely to the in vivo situation, showing a much narrower protein concentrations dynamic range than plasma. In the present chapter, we detail the characterization and analysis of human arterial tissue secretome by two-dimensional electrophoresis (2-DE) and nano-liquid chromatography on-line coupled to mass spectrometry (nLC-MS/MS). General strategies shown here can be extended to other tissue secretome studies.
Original language | English |
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Title of host publication | Vascular Proteomics |
Pages | 81-90 |
Number of pages | 10 |
Volume | 1000 |
DOIs | |
Publication status | Published - 2013 |
Keywords / Materials (for Non-textual outputs)
- Arteries
- Breast
- Chromatography, Liquid
- Coronary Vessels
- Electrophoresis, Gel, Two-Dimensional
- Humans
- Nanotechnology
- Peptide Fragments
- Proteolysis
- Proteomics
- Tandem Mass Spectrometry