Characterization and analysis of human arterial tissue secretome by 2-DE and nLC-MS/MS

Fernando de la Cuesta; Maria G Barderas; Enrique Calvo; Irene Zubiri; Aroa S Maroto; Juan Antonio Lopez; Fernando Vivanco; Gloria Alvarez-Llamas

doi:10.1007/978-1-62703-405-0_7

Characterization and analysis of human arterial tissue secretome by 2-DE and nLC-MS/MS

Fernando de la Cuesta, Maria G Barderas, Enrique Calvo, Irene Zubiri, Aroa S Maroto, Juan Antonio Lopez, Fernando Vivanco, Gloria Alvarez-Llamas

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Early detection of cardiovascular diseases and knowledge of underlying mechanisms is essential. Tissue secretome studies resemble more closely to the in vivo situation, showing a much narrower protein concentrations dynamic range than plasma. In the present chapter, we detail the characterization and analysis of human arterial tissue secretome by two-dimensional electrophoresis (2-DE) and nano-liquid chromatography on-line coupled to mass spectrometry (nLC-MS/MS). General strategies shown here can be extended to other tissue secretome studies.

Original language	English
Title of host publication	Vascular Proteomics
Pages	81-90
Number of pages	10
Volume	1000
DOIs	https://doi.org/10.1007/978-1-62703-405-0_7
Publication status	Published - 2013

Keywords

Arteries
Breast
Chromatography, Liquid
Coronary Vessels
Electrophoresis, Gel, Two-Dimensional
Humans
Nanotechnology
Peptide Fragments
Proteolysis
Proteomics
Tandem Mass Spectrometry

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10.1007/978-1-62703-405-0_7

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title = "Characterization and analysis of human arterial tissue secretome by 2-DE and nLC-MS/MS",

abstract = "Early detection of cardiovascular diseases and knowledge of underlying mechanisms is essential. Tissue secretome studies resemble more closely to the in vivo situation, showing a much narrower protein concentrations dynamic range than plasma. In the present chapter, we detail the characterization and analysis of human arterial tissue secretome by two-dimensional electrophoresis (2-DE) and nano-liquid chromatography on-line coupled to mass spectrometry (nLC-MS/MS). General strategies shown here can be extended to other tissue secretome studies.",

keywords = "Arteries, Breast, Chromatography, Liquid, Coronary Vessels, Electrophoresis, Gel, Two-Dimensional, Humans, Nanotechnology, Peptide Fragments, Proteolysis, Proteomics, Tandem Mass Spectrometry",

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doi = "10.1007/978-1-62703-405-0_7",

language = "English",

volume = "1000",

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TY - CHAP

T1 - Characterization and analysis of human arterial tissue secretome by 2-DE and nLC-MS/MS

AU - de la Cuesta, Fernando

AU - Barderas, Maria G

AU - Calvo, Enrique

AU - Zubiri, Irene

AU - Maroto, Aroa S

AU - Lopez, Juan Antonio

AU - Vivanco, Fernando

AU - Alvarez-Llamas, Gloria

PY - 2013

Y1 - 2013

N2 - Early detection of cardiovascular diseases and knowledge of underlying mechanisms is essential. Tissue secretome studies resemble more closely to the in vivo situation, showing a much narrower protein concentrations dynamic range than plasma. In the present chapter, we detail the characterization and analysis of human arterial tissue secretome by two-dimensional electrophoresis (2-DE) and nano-liquid chromatography on-line coupled to mass spectrometry (nLC-MS/MS). General strategies shown here can be extended to other tissue secretome studies.

AB - Early detection of cardiovascular diseases and knowledge of underlying mechanisms is essential. Tissue secretome studies resemble more closely to the in vivo situation, showing a much narrower protein concentrations dynamic range than plasma. In the present chapter, we detail the characterization and analysis of human arterial tissue secretome by two-dimensional electrophoresis (2-DE) and nano-liquid chromatography on-line coupled to mass spectrometry (nLC-MS/MS). General strategies shown here can be extended to other tissue secretome studies.

KW - Arteries

KW - Breast

KW - Chromatography, Liquid

KW - Coronary Vessels

KW - Electrophoresis, Gel, Two-Dimensional

KW - Humans

KW - Nanotechnology

KW - Peptide Fragments

KW - Proteolysis

KW - Proteomics

KW - Tandem Mass Spectrometry

U2 - 10.1007/978-1-62703-405-0_7

DO - 10.1007/978-1-62703-405-0_7

M3 - Chapter

C2 - 23585086

VL - 1000

SP - 81

EP - 90

BT - Vascular Proteomics

ER -

Characterization and analysis of human arterial tissue secretome by 2-DE and nLC-MS/MS

Abstract

Keywords

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