Abstract / Description of output
A cDNA encoding the bovine dopamine receptor 1 (DRD1) was isolated from a bovine cDNA library, cloned and completely sequenced. The coding region showed 93 and 91% sequence identity on DNA level and 96 and 94% on protein level with its respective porcine and human orthologs. The bovine DRD1 and dopamine receptor 5 (DRD5) were mapped, respectively, to BTA10 and 6 by radiation hybrid mapping. One SNP was found in DRD1 and four in DRD5. Using polymerase chain reaction-restriction fragment length polymorphism. 11 different European cattle breeds were screened for the presence of the DRD1 and DRD5 substitutions. Allele frequencies for DRD1 and DRD5 alleles were very similar across all the breeds examined. Allele frequency discrepancies were found between Belgian Blue beef breed and the other breeds.
Original language | English |
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Pages (from-to) | 290-293 |
Number of pages | 4 |
Journal | Animal Genetics |
Volume | 34 |
Issue number | 4 |
DOIs | |
Publication status | Published - 1 Aug 2003 |
Keywords / Materials (for Non-textual outputs)
- Complementary deoxyribonucleic acid
- Dopamine receptor
- Mutation
- Polymerase chain reaction
- Restriction fragment length polymorphism
- SSCP