Characterization of 24 porcine (dA-dC)n-(dT-dG)n microsatellites: genotyping of unrelated animals from four breeds and linkage studies

M Fredholm, A K Winterø, K Christensen, B Kristensen, P B Nielsen, W Davies, A Archibald

Research output: Contribution to journalArticlepeer-review

Abstract

Twenty-four PCR primer pairs were designed for the detection of porcine microsatellites. Polymorphism was investigated in 76 unrelated animals from four different breeds: Duroc, Landrace, Hampshire, and Yorkshire. Compared with human microsatellites, a general lower heterozygosity was detected; however, for each microsatellite a significant variation between breeds in number of alleles and heterozygosity was seen. Mean heterozygosity was found to be significantly higher (P <0.01%) in the Yorkshire breed than in the other three breeds. Linkage analyses with the CEPH linkage packet were performed in a backcross family comprising 45 animals, of which 43 had informative meioses. Ten of the microsatellites could be assigned to six different linkage groups, demonstrating that linkage mapping with microsatellites can be carried out with great efficiency in a relatively small number of animals. Four of the linkage groups represent Chromosomes (Chrs) 4, 6, 7, and 8 respectively, while two linkage groups are unassigned.
Original languageEnglish
Pages (from-to)187-92
Number of pages6
JournalMammalian Genome
Volume4
Issue number4
Publication statusPublished - 1993

Keywords

  • Alleles
  • Animals
  • Base Sequence
  • Breeding
  • Crosses, Genetic
  • DNA Probes
  • DNA, Satellite
  • Female
  • Genotype
  • Lod Score
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Genetic
  • Recombination, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Species Specificity
  • Swine

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