Characterization of Engineered L1 Retrotransposition Events: The Recovery Method

David Cano, Santiago Morell, Andres J Pulgarin, Suyapa Amador, Jose L Garcia-Pérez

Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution


Long Interspersed Element class 1 retrotransposons (LINE-1 or L1) are abundant Transposable Elements in mammalian genomes and their mobility continues to impact the human genome. The development of engineered retrotransposition assays has been instrumental to understand how these elements are regulated and to identify domains involved in the process of retrotransposition. Additionally, the modification of a retrotransposition indicator cassette has allowed developing straightforward approaches to characterize the site of new L1 insertions in cultured cells. In this chapter, we describe a method termed "L1-recovery" that has been used to characterize the site of insertion on engineered L1 retrotransposition events in cultured mammalian cells. Notably, the recovery assay is based on a genetic strategy and avoids the use of PCR and thus reduces to a minimum the appearance of false positives/artifacts.

Original languageEnglish
Title of host publicationTransposons and Retrotransposons
Number of pages18
Publication statusPublished - 20 Feb 2016

Publication series

NameMethods in Molecular Biology


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