Characterization of microsatellites from flow-sorted porcine chromosome 13

W Davies, B Høyheim, B Chaput, A L Archibald, G Frelat

Research output: Contribution to journalArticlepeer-review

Abstract

Porcine flow-sorted Chromosome (Chr) 13 was PCR amplified with primers based on porcine short interspersed element (SINE) sequences. The product was cloned, gridded in microtiter plates, and screened with a [GT]10 oligonucleotide which gave 45 positive clones. Sequencing of these clones showed that 36 were unique, and 26 [GT]n microsatellites were characterized. Six other simple repeat sequences, the majority of which were associated with the 3' end of the SINE sequence, were also detected. Twenty-one primers sets were selected, and 13 of these detected useful polymorphisms in the grandparents (n = 26) of the European porcine mapping collaboration (PiGMaP) reference families. These 13 markers were mapped in the "PiGMaP" reference families, and a two-point linkage analysis was performed. The Lod scores indicated that three of the markers were not linked and the remaining 11 formed two linkage groups of two and nine markers respectively. The larger linkage group was also linked to the transferrin locus, permitting assignment of nine markers to porcine Chr 13.
Original languageEnglish
Pages (from-to)707-11
Number of pages5
JournalMammalian Genome
Volume5
Issue number11
Publication statusPublished - Nov 1994

Keywords

  • Animals
  • Base Sequence
  • Cell Fractionation
  • Chromosome Mapping
  • Chromosomes
  • DNA Primers
  • DNA, Satellite
  • Flow Cytometry
  • Genetic Markers
  • Lod Score
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Repetitive Sequences, Nucleic Acid
  • Swine

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