Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines: E-cadherin/catenin complex and colorectal carcinoma

Mona El-bahrawy, S. Richard Poulsom, Andrew J. Rowan, Ian T. Tomlinson, Malcolm R. Alison

Research output: Contribution to journalArticlepeer-review

Abstract

The E-cadherin/catenin complex is a prime mediator of cell–cell adhesion. APC mutations can result in loss of β-catenin downregulation and an accumulation of β-catenin in the cell. β-CATENIN mutations can have a similar effect. The aim of this study was to investigate the effect of β-CATENIN and APC mutations on the expression and assembly of the E-cadherin/catenin complex. Five colorectal carcinoma cell lines with different APC and β-CATENIN gene status were selected and mutations were confirmed. The expression of members of the E-cadherin/catenin complex was studied by immunohistochemistry and Western blotting. Complex assembly was investigated by immunoprecipitation. It is shown that E-cadherin and catenins are expressed in colorectal carcinoma cell lines with the predominant complex assembly being E-cadherin/β-catenin/α-catenin. The subcellular distribution of the proteins is influenced by cell–cell contact, resulting in membranous localization. The expression and assembly of the E-cadherin/catenin complex does not appear to be affected by the presence of APC and or β-CATENIN mutations
Original languageEnglish
Pages (from-to)65-74
JournalInternational Journal of Experimental Pathology
Volume85
Issue number2
DOIs
Publication statusPublished - Apr 2004

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