Characterization of the feline thyroglobulin promoter

L Blackwood, D E Onions, D J Argyle

Research output: Contribution to journalArticlepeer-review

Abstract

The feline thyroglobulin promoter was identified by a combination of standard polymerase chain reaction (PCR) techniques, using primers designed according to regions of homology in published sequences from other species, then adaptor ligated PCR. A 310 bp fragment of the feline thyroglobulin promoter was generated, including 8 nucleotides of adaptor sequence at the 5' end and, based on the putative transcription start site, 36 nucleotides of the thyroglobulin mRNA (untranslated portion). The homology between the feline promoter sequence (from 193 bp upstream to the putative cap site) and canine, bovine and human sequences was 89%, 81% and 78%, respectively. Transient transfection studies, using reporter constructs in which the feline promoter controlled expression of chloramphenicol acetyl transferase, demonstrated promoter activity in thyroid cells, but no activity in non-thyroid cells. The data presented here demonstrate that the feline thyroglobulin promoter may provide a targeting mechanism for somatic gene therapy of feline thyroid disease.
Original languageEnglish
Pages (from-to)185-201
Number of pages17
JournalDomestic Animal Endocrinology
Volume20
Issue number3
Publication statusPublished - Apr 2001

Keywords

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cats
  • Cattle
  • Cell Line
  • DNA
  • Dogs
  • Gene Expression
  • Humans
  • Mice
  • Molecular Sequence Data
  • Organ Specificity
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Rats
  • Sequence Homology
  • Thyroglobulin
  • Thyroid Gland
  • Transfection

Fingerprint

Dive into the research topics of 'Characterization of the feline thyroglobulin promoter'. Together they form a unique fingerprint.

Cite this