Characterization of the fine specificity of bovine CD8 T-cell responses to defined antigens from the protozoan parasite Theileria parva

S. P. Graham, R. Pelle, M. Yamage, D. M. Mwangi, Y. Honda, R. S. Mwakubambanya, E. P. de Villiers, E. Abuya, E. Awino, J. Gachanja, F. Mbwika, A. M. Muthiani, C. Muriuki, J. K. Nyanjui, F. O. Onono, J. Osaso, V. Riitho, R. M. Saya, S. A. Ellis, D. J. McKeeverN. D. MacHugh, S. C. Gilbert, J. C. Audonnet, W. I. Morrison, P. van der Bruggen, E. L. Taracha

Research output: Contribution to journalArticlepeer-review


Immunity against the bovine intracellular protozoan parasite Theileria parva has been shown to be mediated
by CD8 T cells. Six antigens targeted by CD8 T cells from T. parva-immune cattle of different major histocompatibility complex (MHC) genotypes have been identified, raising the prospect of developing a subunit
vaccine. To facilitate further dissection of the specificity of protective CD8 T-cell responses and to assist in the
assessment of responses to vaccination, we set out to identify the epitopes recognized in these T. parva antigens
and their MHC restriction elements. Nine epitopes in six T. parva antigens, together with their respective MHC
restriction elements, were successfully identified. Five of the cytotoxic-T-lymphocyte epitopes were found to be
restricted by products of previously described alleles, and four were restricted by four novel restriction
elements. Analyses of CD8 T-cell responses to five of the epitopes in groups of cattle carrying the defined
restriction elements and immunized with live parasites demonstrated that, with one exception, the epitopes
were consistently recognized by animals of the respective genotypes. The analysis of responses was extended to
animals immunized with multiple antigens delivered in separate vaccine constructs. Specific CD8 T-cell
responses were detected in 19 of 24 immunized cattle. All responder cattle mounted responses specific for
antigens for which they carried an identified restriction element. By contrast, only 8 of 19 responder cattle
displayed a response to antigens for which they did not carry an identified restriction element. These data
demonstrate that the identified antigens are inherently dominant in animals with the corresponding MHC
Original languageEnglish
Pages (from-to)685-694
Number of pages10
JournalInfection and Immunity
Issue number2
Publication statusPublished - Feb 2008


Dive into the research topics of 'Characterization of the fine specificity of bovine CD8 T-cell responses to defined antigens from the protozoan parasite Theileria parva'. Together they form a unique fingerprint.

Cite this