Characterization of unconventional kinetochore kinases KKT10 and KKT19 in Trypanosoma brucei

Midori Ishii, Bungo Akiyoshi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The kinetochore is a macromolecular protein complex that drives chromosome segregation in eukaryotes. Unlike most eukaryotes that have canonical kinetochore proteins, evolutionarily divergent kinetoplastids, such as Trypanosoma brucei, have unconventional kinetochore proteins. T. brucei also lacks a canonical spindle checkpoint system, and it therefore remains unknown how mitotic progression is regulated in this organism. Here, we characterized, in the procyclic form of T. brucei, two paralogous kinetochore proteins with a CLK-like kinase domain, KKT10 and KKT19, which localize at kinetochores in metaphase but disappear at the onset of anaphase. We found that these proteins are functionally redundant. Double knockdown of KKT10 and KKT19 led to a significant delay in the metaphase to anaphase transition. We also found that phosphorylation of two kinetochore proteins, KKT4 and KKT7, depended on KKT10 and KKT19 in vivo. Finally, we showed that the N-terminal part of KKT7 directly interacts with KKT10 and that kinetochore localization of KKT10 depends not only on KKT7 but also on the KKT8 complex. Our results reveal that kinetochore localization of KKT10 and KKT19 is tightly controlled to regulate the metaphase to anaphase transition in T. brucei.

Original languageEnglish
Article numberjcs240978
Number of pages12
JournalJournal of Cell Science
Issue number8
Publication statusPublished - 29 Apr 2020
Externally publishedYes

Keywords / Materials (for Non-textual outputs)

  • Kinetoplastid kinetochore
  • Trypanosoma brucei
  • Kinase
  • KKT
  • Cell cycle


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