Characterizing the phenotype of murine epidermal progenitor cells: complementary whole-mount visualization and flow cytometry strategies

Korinna D Henseleit, Ann P Wheeler, Gary Warnes, Kristin M Braun

Research output: Contribution to journalArticlepeer-review

Abstract

The epidermis and its appendages, the hair follicle and sebaceous gland, have the capacity to constantly regenerate throughout adult life. Postnatal hair follicles undergo a cyclic mode of tissue homeostasis, defined by periods of growth, degeneration, and rest. A multipotent population of stem cells residing within the hair follicle bulge not only generates the hair lineages during each hair cycle, but also transiently contributes to the repair of epidermis following wounding. In this chapter, we provide methods for identifying epidermal stem cells and investigating their proliferative and apoptotic characteristics. We introduce whole-mount and flow cytometry techniques, which complement each other by permitting visualization of the epidermal stem cell compartment in situ and assessment of the phenotype of purified cells. These techniques can easily be adapted to characterize novel putative epidermal stem or progenitor cell populations. By applying whole-mount and flow cytometry techniques to characterize normal and genetically modified mice with skin defects, we expect to learn more about the factors that regulate stem cell self-renewal and differentiation.

Original languageEnglish
Pages (from-to)243-61
Number of pages19
JournalMethods in Molecular Biology
Volume916
DOIs
Publication statusPublished - 2012

Keywords

  • Animals
  • Annexin A5
  • Apoptosis
  • Cell Cycle
  • Epidermis
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Mice
  • Microscopy, Confocal
  • Molecular Imaging
  • Phenotype
  • Staining and Labeling
  • Stem Cells

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