Chemical evolution of a bacterial proteome

Michael Georg Hoesl; Stefan Oehm; Patrick Durkin; Elise Darmon; Lauri Peil; Hans-Rudolf Aerni; Juri Rappsilber; Jesse Rinehart; David Leach; Dieter Söll; Nediljko Budisa

doi:10.1002/anie.201502868

Chemical evolution of a bacterial proteome

Michael Georg Hoesl, Stefan Oehm, Patrick Durkin, Elise Darmon, Lauri Peil, Hans-Rudolf Aerni, Juri Rappsilber, Jesse Rinehart, David Leach, Dieter Söll, Nediljko Budisa

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

We have changed the amino acid set of the genetic code of Escherichia coli by evolving cultures capable of growing on the synthetic noncanonical amino acid L-β-(thieno[3,2-b]pyrrolyl)alanine ([3,2]Tpa) as a sole surrogate for the canonical amino acid L-tryptophan (Trp). A long-term cultivation experiment in defined synthetic media resulted in the evolution of cells capable of surviving Trp→[3,2]Tpa substitutions in their proteomes in response to the 20,899 TGG codons of the E. coli W3110 genome. These evolved bacteria with new-to-nature amino acid composition showed robust growth in the complete absence of Trp. Our experimental results illustrate an approach for the evolution of synthetic cells with alternative biochemical building blocks.

Original language	English
Pages (from-to)	10030-10034
Number of pages	5
Journal	Angewandte Chemie International Edition
Volume	54
Issue number	34
Early online date	1 Jul 2015
DOIs	https://doi.org/10.1002/anie.201502868
Publication status	Published - 17 Aug 2015

Keywords / Materials (for Non-textual outputs)

alanine
bridged bicyclo compounds
escherichia coli
escherichia coli proteins
evolution
proteome
chemical
heterocyclic
analogs & derivatives

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10.1002/anie.201502868

Elise Darmon
- School of Biological Sciences - Lecturer in Molecular Bioscience Education
Person: Academic: Research Active (Research Assistant)

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title = "Chemical evolution of a bacterial proteome",

abstract = "We have changed the amino acid set of the genetic code of Escherichia coli by evolving cultures capable of growing on the synthetic noncanonical amino acid L-β-(thieno[3,2-b]pyrrolyl)alanine ([3,2]Tpa) as a sole surrogate for the canonical amino acid L-tryptophan (Trp). A long-term cultivation experiment in defined synthetic media resulted in the evolution of cells capable of surviving Trp→[3,2]Tpa substitutions in their proteomes in response to the 20,899 TGG codons of the E. coli W3110 genome. These evolved bacteria with new-to-nature amino acid composition showed robust growth in the complete absence of Trp. Our experimental results illustrate an approach for the evolution of synthetic cells with alternative biochemical building blocks.",

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T1 - Chemical evolution of a bacterial proteome

AU - Hoesl, Michael Georg

AU - Oehm, Stefan

AU - Durkin, Patrick

AU - Darmon, Elise

AU - Peil, Lauri

AU - Aerni, Hans-Rudolf

AU - Rappsilber, Juri

AU - Rinehart, Jesse

AU - Leach, David

AU - Söll, Dieter

AU - Budisa, Nediljko

PY - 2015/8/17

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AB - We have changed the amino acid set of the genetic code of Escherichia coli by evolving cultures capable of growing on the synthetic noncanonical amino acid L-β-(thieno[3,2-b]pyrrolyl)alanine ([3,2]Tpa) as a sole surrogate for the canonical amino acid L-tryptophan (Trp). A long-term cultivation experiment in defined synthetic media resulted in the evolution of cells capable of surviving Trp→[3,2]Tpa substitutions in their proteomes in response to the 20,899 TGG codons of the E. coli W3110 genome. These evolved bacteria with new-to-nature amino acid composition showed robust growth in the complete absence of Trp. Our experimental results illustrate an approach for the evolution of synthetic cells with alternative biochemical building blocks.

KW - alanine

KW - bridged bicyclo compounds

KW - escherichia coli

KW - escherichia coli proteins

KW - evolution

KW - proteome

KW - chemical

KW - heterocyclic

KW - analogs & derivatives

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DO - 10.1002/anie.201502868

M3 - Article

C2 - 26136259

SN - 1433-7851

VL - 54

SP - 10030

EP - 10034

JO - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

IS - 34

ER -

Chemical evolution of a bacterial proteome

Abstract

Keywords / Materials (for Non-textual outputs)

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Elise Darmon

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