Chemiluminescence of asbestos-activated macrophages

Ken Donaldson, R T Cullen

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Chemiluminescence, a measure of reactive oxygen release by phagocytes, was compared in peritoneal exudate macrophages elicited with chrysotile asbestos, Corynebacterium parvum and saline. Chrysotile asbestos- and C. parvum-activated macrophages produced significantly more chemiluminescence than saline-elicited macrophages. In a second series of experiments the ability of opsonized chrysotile asbestos to act as a trigger for the release of chemiluminescence was tested. Opsonized chrysotile asbestos produced a dose-related release of chemiluminescence from activated macrophages except at the highest dose where chemiluminescence was reduced due, possibly, to a toxic effect of chrysotile during the assay. Opsonized latex also triggered a dose-related chemiluminescent response from activated macrophages. The potential role of toxic reactive oxygen species, released from macrophages, in the development of asbestos-related pulmonary inflammation and fibrosis are discussed.
Original languageEnglish
Pages (from-to)81-90
Number of pages10
JournalBritish journal of experimental pathology
Volume65
Issue number1
Publication statusPublished - 1984

Keywords / Materials (for Non-textual outputs)

  • Chemiluminescence
  • asbestos-activated macrophages

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