Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs

Debin Tian, Yan-Yan Ni, Lei Zhou, Tanja Opriessnig, Dianjun Cao, Pablo Piñeyro, Danielle M Yugo, Christopher Overend, Qian Cao, C Lynn Heffron, Patrick G Halbur, Douglas S Pearce, Jay G Calvert, Xiang-Jin Meng

Research output: Contribution to journalArticlepeer-review

Abstract

The extensive genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains is a major obstacle for vaccine development. We previously demonstrated that chimeric PRRSVs in which a single envelope gene (ORF3, ORF4, ORF5 or ORF6) was shuffled via DNA shuffling had an improved heterologous cross-neutralizing ability. In this study, we incorporate all of the individually-shuffled envelope genes together in different combinations into an infectious clone backbone of PRRSV MLV Fostera(®) PRRS. Five viable progeny chimeric viruses were rescued, and their growth characteristics were characterized in vitro. In a pilot pig study, two chimeric viruses (FV-SPDS-VR2,FV-SPDS-VR5) were found to induce cross-neutralizing antibodies against heterologous strains. A subsequent vaccination/challenge study in 72 pigs revealed that chimeric virus FV-SPDS-VR2 and parental virus conferred partial cross-protection when challenged with heterologous strains NADC20 or MN184B. The results have important implications for future development of an effective PRRSV vaccine that confers heterologous protection.

Original languageEnglish
Pages (from-to)402-413
Number of pages12
JournalVirology
Volume485
DOIs
Publication statusPublished - 2 Sep 2015

Fingerprint

Dive into the research topics of 'Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs'. Together they form a unique fingerprint.

Cite this