Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs

Debin Tian; Yan-Yan Ni; Lei Zhou; Tanja Opriessnig; Dianjun Cao; Pablo Piñeyro; Danielle M Yugo; Christopher Overend; Qian Cao; C Lynn Heffron; Patrick G Halbur; Douglas S Pearce; Jay G Calvert; Xiang-Jin Meng

doi:10.1016/j.virol.2015.08.021

Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs

Debin Tian, Yan-Yan Ni, Lei Zhou, Tanja Opriessnig, Dianjun Cao, Pablo Piñeyro, Danielle M Yugo, Christopher Overend, Qian Cao, C Lynn Heffron, Patrick G Halbur, Douglas S Pearce, Jay G Calvert, Xiang-Jin Meng

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The extensive genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains is a major obstacle for vaccine development. We previously demonstrated that chimeric PRRSVs in which a single envelope gene (ORF3, ORF4, ORF5 or ORF6) was shuffled via DNA shuffling had an improved heterologous cross-neutralizing ability. In this study, we incorporate all of the individually-shuffled envelope genes together in different combinations into an infectious clone backbone of PRRSV MLV Fostera(®) PRRS. Five viable progeny chimeric viruses were rescued, and their growth characteristics were characterized in vitro. In a pilot pig study, two chimeric viruses (FV-SPDS-VR2,FV-SPDS-VR5) were found to induce cross-neutralizing antibodies against heterologous strains. A subsequent vaccination/challenge study in 72 pigs revealed that chimeric virus FV-SPDS-VR2 and parental virus conferred partial cross-protection when challenged with heterologous strains NADC20 or MN184B. The results have important implications for future development of an effective PRRSV vaccine that confers heterologous protection.

Original language	English
Pages (from-to)	402-413
Number of pages	12
Journal	Virology
Volume	485
DOIs	https://doi.org/10.1016/j.virol.2015.08.021
Publication status	Published - 2 Sept 2015

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Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigsAccepted author manuscript, 1.43 MB

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Tian, D., Ni, Y.-Y., Zhou, L., Opriessnig, T., Cao, D., Piñeyro, P., Yugo, D. M., Overend, C., Cao, Q., Lynn Heffron, C., Halbur, P. G., Pearce, D. S., Calvert, J. G., & Meng, X.-J. (2015). Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs. Virology, 485, 402-413. https://doi.org/10.1016/j.virol.2015.08.021

@article{478c146c610c4804addd41fd9b271069,

title = "Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs",

abstract = "The extensive genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains is a major obstacle for vaccine development. We previously demonstrated that chimeric PRRSVs in which a single envelope gene (ORF3, ORF4, ORF5 or ORF6) was shuffled via DNA shuffling had an improved heterologous cross-neutralizing ability. In this study, we incorporate all of the individually-shuffled envelope genes together in different combinations into an infectious clone backbone of PRRSV MLV Fostera({\textregistered}) PRRS. Five viable progeny chimeric viruses were rescued, and their growth characteristics were characterized in vitro. In a pilot pig study, two chimeric viruses (FV-SPDS-VR2,FV-SPDS-VR5) were found to induce cross-neutralizing antibodies against heterologous strains. A subsequent vaccination/challenge study in 72 pigs revealed that chimeric virus FV-SPDS-VR2 and parental virus conferred partial cross-protection when challenged with heterologous strains NADC20 or MN184B. The results have important implications for future development of an effective PRRSV vaccine that confers heterologous protection.",

author = "Debin Tian and Yan-Yan Ni and Lei Zhou and Tanja Opriessnig and Dianjun Cao and Pablo Pi{\~n}eyro and Yugo, {Danielle M} and Christopher Overend and Qian Cao and {Lynn Heffron}, C and Halbur, {Patrick G} and Pearce, {Douglas S} and Calvert, {Jay G} and Xiang-Jin Meng",

year = "2015",

month = sep,

day = "2",

doi = "10.1016/j.virol.2015.08.021",

language = "English",

volume = "485",

pages = "402--413",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press",

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Tian, D, Ni, Y-Y, Zhou, L, Opriessnig, T, Cao, D, Piñeyro, P, Yugo, DM, Overend, C, Cao, Q, Lynn Heffron, C, Halbur, PG, Pearce, DS, Calvert, JG & Meng, X-J 2015, 'Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs', Virology, vol. 485, pp. 402-413. https://doi.org/10.1016/j.virol.2015.08.021

TY - JOUR

T1 - Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs

AU - Tian, Debin

AU - Ni, Yan-Yan

AU - Zhou, Lei

AU - Opriessnig, Tanja

AU - Cao, Dianjun

AU - Piñeyro, Pablo

AU - Yugo, Danielle M

AU - Overend, Christopher

AU - Cao, Qian

AU - Lynn Heffron, C

AU - Halbur, Patrick G

AU - Pearce, Douglas S

AU - Calvert, Jay G

AU - Meng, Xiang-Jin

PY - 2015/9/2

Y1 - 2015/9/2

N2 - The extensive genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains is a major obstacle for vaccine development. We previously demonstrated that chimeric PRRSVs in which a single envelope gene (ORF3, ORF4, ORF5 or ORF6) was shuffled via DNA shuffling had an improved heterologous cross-neutralizing ability. In this study, we incorporate all of the individually-shuffled envelope genes together in different combinations into an infectious clone backbone of PRRSV MLV Fostera(®) PRRS. Five viable progeny chimeric viruses were rescued, and their growth characteristics were characterized in vitro. In a pilot pig study, two chimeric viruses (FV-SPDS-VR2,FV-SPDS-VR5) were found to induce cross-neutralizing antibodies against heterologous strains. A subsequent vaccination/challenge study in 72 pigs revealed that chimeric virus FV-SPDS-VR2 and parental virus conferred partial cross-protection when challenged with heterologous strains NADC20 or MN184B. The results have important implications for future development of an effective PRRSV vaccine that confers heterologous protection.

AB - The extensive genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains is a major obstacle for vaccine development. We previously demonstrated that chimeric PRRSVs in which a single envelope gene (ORF3, ORF4, ORF5 or ORF6) was shuffled via DNA shuffling had an improved heterologous cross-neutralizing ability. In this study, we incorporate all of the individually-shuffled envelope genes together in different combinations into an infectious clone backbone of PRRSV MLV Fostera(®) PRRS. Five viable progeny chimeric viruses were rescued, and their growth characteristics were characterized in vitro. In a pilot pig study, two chimeric viruses (FV-SPDS-VR2,FV-SPDS-VR5) were found to induce cross-neutralizing antibodies against heterologous strains. A subsequent vaccination/challenge study in 72 pigs revealed that chimeric virus FV-SPDS-VR2 and parental virus conferred partial cross-protection when challenged with heterologous strains NADC20 or MN184B. The results have important implications for future development of an effective PRRSV vaccine that confers heterologous protection.

U2 - 10.1016/j.virol.2015.08.021

DO - 10.1016/j.virol.2015.08.021

M3 - Article

C2 - 26342466

SN - 0042-6822

VL - 485

SP - 402

EP - 413

JO - Virology

JF - Virology

ER -

Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs

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