Chip-on-tip FLIm micro-camera towards endoscopic applications

Dario Angelone; Noreen Nudds; Andrew Matheson; Ahmet Erdogan; Walter Messina; Ray Burke; Robert K. Henderson; Stefan Andersson-Engels; Sanathana Konugolu Venkata Sekar

doi:10.1364/OL.561838

Chip-on-tip FLIm micro-camera towards endoscopic applications

Dario Angelone, Noreen Nudds, Andrew Matheson, Ahmet Erdogan, Walter Messina, Ray Burke, Robert K. Henderson, Stefan Andersson-Engels, Sanathana Konugolu Venkata Sekar

School of Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

We present a chip-on-tip fluorescence lifetime imaging micro-camera on a size scale compatible with endoscopic applications. The micro-camera has an external diameter of 4 mm and comprises a miniaturized 128 × 120 SPAD array detector, integrated imaging optics, and an optical fiber for excitation light delivery. The spatial performance is characterized by imaging a resolution test target showing that the integrated system is able to resolve features of <30 µm. The temporal response is measured at a range of wavelengths, yielding a mean pixel impulse response width of < 1 ns. Benchmarking against a time-correlated single-photon counting system also shows good agreement with the micro-camera. Finally, fluorescence lifetime images of unlabeled ex vivo animal tissue samples are presented as examples.

Original language	English
Pages (from-to)	4230-4233
Number of pages	4
Journal	Optics Letters
Volume	50
Issue number	13
Early online date	20 Jun 2025
DOIs	https://doi.org/10.1364/OL.561838
Publication status	Published - 1 Jul 2025

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10.1364/OL.561838Licence: Creative Commons: Attribution (CC-BY)

https://opg.optica.org/ol/fulltext.cfm?uri=ol-50-13-4230&id=573127Licence: Creative Commons: Attribution (CC-BY)

EPSRC IRC Proteus - Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging - Lifetime and Beyond
Bradley, M. (Principal Investigator), Bradley, M. (Principal Investigator), Dhaliwal, K. (Co-investigator), Dhaliwal, K. (Co-investigator), Haslett, C. (Co-investigator), Henderson, R. (Co-investigator), Walsh, T. (Co-investigator) & Walsh, T. (Co-investigator)
Engineering and Physical Sciences Research Council
1/01/19 → 1/06/23
Project: Research

Cite this

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title = "Chip-on-tip FLIm micro-camera towards endoscopic applications",

abstract = "We present a chip-on-tip fluorescence lifetime imaging micro-camera on a size scale compatible with endoscopic applications. The micro-camera has an external diameter of 4 mm and comprises a miniaturized 128 × 120 SPAD array detector, integrated imaging optics, and an optical fiber for excitation light delivery. The spatial performance is characterized by imaging a resolution test target showing that the integrated system is able to resolve features of <30 µm. The temporal response is measured at a range of wavelengths, yielding a mean pixel impulse response width of < 1 ns. Benchmarking against a time-correlated single-photon counting system also shows good agreement with the micro-camera. Finally, fluorescence lifetime images of unlabeled ex vivo animal tissue samples are presented as examples.",

author = "Dario Angelone and Noreen Nudds and Andrew Matheson and Ahmet Erdogan and Walter Messina and Ray Burke and Henderson, \{Robert K.\} and Stefan Andersson-Engels and \{Konugolu Venkata Sekar\}, Sanathana",

year = "2025",

month = jul,

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doi = "10.1364/OL.561838",

language = "English",

volume = "50",

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T1 - Chip-on-tip FLIm micro-camera towards endoscopic applications

AU - Angelone, Dario

AU - Nudds, Noreen

AU - Matheson, Andrew

AU - Erdogan, Ahmet

AU - Messina, Walter

AU - Burke, Ray

AU - Henderson, Robert K.

AU - Andersson-Engels, Stefan

AU - Konugolu Venkata Sekar, Sanathana

PY - 2025/7/1

Y1 - 2025/7/1

N2 - We present a chip-on-tip fluorescence lifetime imaging micro-camera on a size scale compatible with endoscopic applications. The micro-camera has an external diameter of 4 mm and comprises a miniaturized 128 × 120 SPAD array detector, integrated imaging optics, and an optical fiber for excitation light delivery. The spatial performance is characterized by imaging a resolution test target showing that the integrated system is able to resolve features of <30 µm. The temporal response is measured at a range of wavelengths, yielding a mean pixel impulse response width of < 1 ns. Benchmarking against a time-correlated single-photon counting system also shows good agreement with the micro-camera. Finally, fluorescence lifetime images of unlabeled ex vivo animal tissue samples are presented as examples.

AB - We present a chip-on-tip fluorescence lifetime imaging micro-camera on a size scale compatible with endoscopic applications. The micro-camera has an external diameter of 4 mm and comprises a miniaturized 128 × 120 SPAD array detector, integrated imaging optics, and an optical fiber for excitation light delivery. The spatial performance is characterized by imaging a resolution test target showing that the integrated system is able to resolve features of <30 µm. The temporal response is measured at a range of wavelengths, yielding a mean pixel impulse response width of < 1 ns. Benchmarking against a time-correlated single-photon counting system also shows good agreement with the micro-camera. Finally, fluorescence lifetime images of unlabeled ex vivo animal tissue samples are presented as examples.

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DO - 10.1364/OL.561838

M3 - Article

SN - 0146-9592

VL - 50

SP - 4230

EP - 4233

JO - Optics Letters

JF - Optics Letters

IS - 13

ER -

Chip-on-tip FLIm micro-camera towards endoscopic applications

Abstract

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Projects

EPSRC IRC Proteus - Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging - Lifetime and Beyond

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