Projects per year
Abstract
Histone modifications and transcription factor-DNA interactions regulate vital processes such as transcription, recombination, repair, and accurate chromosome segregation. Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) has been instrumental in studying genome-wide distribution of DNA-bound or chromatin-associated factors and histone posttranslational modifications (PTMs). Here, we describe a ChIPmentation protocol adapted for fission yeast, Schizosaccharomyces pombe. This method merges Tn5 mediated tagmentation with existing ChIP protocols, resulting in lower sample input requirements with significant reduction in hands-on time and sample preparation costs.
Original language | English |
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Title of host publication | Schizosaccharomyces pombe |
Subtitle of host publication | Methods and Protocols |
Editors | Ruben C. Petreaca |
Place of Publication | New York, NY |
Publisher | Humana Press |
Chapter | 18 |
Pages | 255-266 |
Number of pages | 12 |
Volume | 2862 |
ISBN (Electronic) | 978-1-0716-4168-2 |
ISBN (Print) | 978-1-0716-4167-5 |
DOIs | |
Publication status | E-pub ahead of print - 12 Nov 2024 |
Publication series
Name | Methods in Molecular Biology |
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Publisher | Humana, New York, NY |
Volume | 2862 |
Keywords / Materials (for Non-textual outputs)
- ChIPmentation
- ChIP-Seq
- Protein-DNA interactions
- Nucleosome
- Histone modifications
- Heterochromatin
- CENP-ACnp1
- Fission yeast
Fingerprint
Dive into the research topics of 'ChIPmentation for Epigenomic Analysis in Fission Yeast'. Together they form a unique fingerprint.-
The Hidden Cell Discovery Research Platform
Marston, A., Allshire, R., Davies, O., El Karoui, M., Kustatscher, G., O'Carroll, D., Rappsilber, J. & Rosser, S.
1/12/23 → 30/11/30
Project: Research
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�Core Funding for the Wellcome Trust Centre for Cell Biology�, Research Enrichment, Public Engagement
1/12/18 → 30/11/24
Project: Research
Research output
- 1 Chapter
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Genome-Wide Profiling of Histone Modifications in Fission Yeast Using CUT&Tag
Torres-Garcia, S., Huang, Y., Dewornu, F. S., Tong, P., Yeboah, R., Allshire, R. & Shukla, M., 12 Nov 2024, (E-pub ahead of print) Schizosaccharomyces pombe: Methods and Protocols. Petreaca, R. C. (ed.). New York, NY: Humana Press, Vol. 2862. p. 309-320 12 p. (Methods in Molecular Biology; vol. 2862).Research output: Chapter in Book/Report/Conference proceeding › Chapter