ChIPmentation for Epigenomic Analysis in Fission Yeast

Felix Selasi Dewornu, Pin Tong, Sito Torres-Garcia, Alison Pidoux, Robin Allshire, Manu Shukla*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Histone modifications and transcription factor-DNA interactions regulate vital processes such as transcription, recombination, repair, and accurate chromosome segregation. Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) has been instrumental in studying genome-wide distribution of DNA-bound or chromatin-associated factors and histone posttranslational modifications (PTMs). Here, we describe a ChIPmentation protocol adapted for fission yeast, Schizosaccharomyces pombe. This method merges Tn5 mediated tagmentation with existing ChIP protocols, resulting in lower sample input requirements with significant reduction in hands-on time and sample preparation costs.
Original languageEnglish
Title of host publicationSchizosaccharomyces pombe
Subtitle of host publicationMethods and Protocols
EditorsRuben C. Petreaca
Place of PublicationNew York, NY
PublisherHumana Press
Chapter18
Pages255-266
Number of pages12
Volume2862
ISBN (Electronic)978-1-0716-4168-2
ISBN (Print)978-1-0716-4167-5
DOIs
Publication statusE-pub ahead of print - 12 Nov 2024

Publication series

NameMethods in Molecular Biology
PublisherHumana, New York, NY
Volume2862

Keywords / Materials (for Non-textual outputs)

  • ChIPmentation
  • ChIP-Seq
  • Protein-DNA interactions
  • Nucleosome
  • Histone modifications
  • Heterochromatin
  • CENP-ACnp1
  • Fission yeast

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  • Genome-Wide Profiling of Histone Modifications in Fission Yeast Using CUT&Tag

    Torres-Garcia, S., Huang, Y., Dewornu, F. S., Tong, P., Yeboah, R., Allshire, R. & Shukla, M., 12 Nov 2024, (E-pub ahead of print) Schizosaccharomyces pombe: Methods and Protocols. Petreaca, R. C. (ed.). New York, NY: Humana Press, Vol. 2862. p. 309-320 12 p. (Methods in Molecular Biology; vol. 2862).

    Research output: Chapter in Book/Report/Conference proceedingChapter

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