Chitosan permeabilizes the plasma membrane and kills cells of Neurospora crassa in an energy dependent manner

J. Palma-Guerrero, I. C. Huang, H. B. Jansson, J. Salinas, L. V. Lopez-Llorca, N. D. Read

Research output: Contribution to journalArticlepeer-review

Abstract

Chitosan has been reported to inhibit spore germination and mycelial growth in plant pathogens, but its mode of antifungal action is poorly understood. Following chitosan treatment. we characterized plasma membrane permeabilization, and cell death and lysis in the experimental model, Neurospora crassa. Rhodamine-labeled chitosan was used to show that chitosan is internalized by fungal cells. Cell viability stains and the calcium reporter, aequorin, were used to monitor plasma membrane permeabilization and cell death. Chitosan permeabilization of the fungal plasma membrane and its uptake into fungal cells was found to be energy dependent but not to involve endocytosis. Different cell types (conidia, germ tubes and vegetative hyphae) exhibited differential sensitivity to chitosan with ungerminated conidia being the most sensitive.
Original languageEnglish
Pages (from-to)585-594
JournalFungal Genetics and Biology
Volume46
DOIs
Publication statusPublished - 2009

Keywords

  • Aequorin
  • Conidial anastomosis tube
  • Fungicide
  • Hyphal fusion
  • Live-cell imaging

Fingerprint

Dive into the research topics of 'Chitosan permeabilizes the plasma membrane and kills cells of Neurospora crassa in an energy dependent manner'. Together they form a unique fingerprint.

Cite this