Chromatin structures of goat and sheep beta-lactoglobulin gene differ

R N Pena, J M Folch, A Sánchez, C B Whitelaw

Research output: Contribution to journalArticlepeer-review

Abstract

Different levels of the major milk protein beta-lactoglobulin are found in evolutionarily related ruminant species: with sheep milk containing as much as three times the concentration in goat milk. In an attempt to understand why these differences exist, we have characterised, using DNaseI as a probe of structure, the chromatin surrounding the goat beta-lactoglobulin promoter and compared it to that of the sheep homologue. The goat gene displays a mammary-specific chromatin pattern, which is reformed on expressing goat beta-lactoglobulin transgenes. This implies that this chromatin structure is sequence dependent and suggests that it plays a role in regulating beta-lactoglobulin gene expression. This pattern differs from that seen on the ovine beta-lactoglobulin gene in lactating sheep mammary chromatin. Thus, even between highly related species, the transcriptional mechanisms regulating activity of a gene can differ.
Original languageEnglish
Pages (from-to)649-53
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume252
Issue number3
DOIs
Publication statusPublished - 27 Nov 1998

Keywords

  • Animals
  • Cell Line
  • Chromatin
  • DNA Fingerprinting
  • Female
  • Goats
  • Lactoglobulins
  • Liver
  • Mammary Glands, Animal
  • Mice
  • Mice, Transgenic
  • Sheep
  • Species Specificity

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