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Abstract / Description of output
Defects in DNA mismatch repair (MMR) result in elevated mutagenesis and in cancer predisposition. This disease burden arises because MMR is required to correct errors made in the copying of DNA. MMR is bidirectional at the level of DNA strand polarity as it operates equally well in the 5' to 3' and the 3' to 5' directions. However, the directionality of MMR with respect to the chromosome, which comprises parental DNA strands of opposite polarity, has been unknown. Here, we show that MMR in Escherichia coli is unidirectional with respect to the chromosome. Our data demonstrate that, following the recognition of a 3-bp insertion-deletion loop mismatch, the MMR machinery searches for the first hemimethylated GATC site located on its origin-distal side, toward the replication fork, and that resection then proceeds back toward the mismatch and away from the replication fork. This study provides support for a tight coupling between MMR and DNA replication.
Original language | English |
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Pages (from-to) | 9388-93 |
Number of pages | 6 |
Journal | Proceedings of the National Academy of Sciences (PNAS) |
Volume | 112 |
Issue number | 30 |
Early online date | 13 Jul 2015 |
DOIs | |
Publication status | Published - 28 Jul 2015 |
Keywords / Materials (for Non-textual outputs)
- E. coli
- mismatch repair
- recombination
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Dive into the research topics of 'Chromosomal directionality of DNA mismatch repair in Escherichia coli'. Together they form a unique fingerprint.Projects
- 1 Finished
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Maintaining Genome Stability: Genetic Recombination, DNA Repair and Chromosome Biology Initiated by DNA Misfolding
Leach, D.
1/07/10 → 30/06/15
Project: Research