Chromosome-level assembly of the common lizard (zootoca vivipara) genome

Andrey A Yurchenko, Hans Recknagel, Kathryn R Elmer

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Squamate reptiles exhibit high variation in their phenotypic traits and geographical distributions and are therefore fascinating taxa for evolutionary and ecological research. However, genomic resources are very limited for this group of species, consequently inhibiting research efforts. To address this gap, we assembled a high-quality genome of the common lizard, Zootoca vivipara (Lacertidae), using a combination of high coverage Illumina (shotgun and mate-pair) and PacBio sequencing data, coupled with RNAseq data and genetic linkage map generation. The 1.46-Gb genome assembly has a scaffold N50 of 11.52 Mb with N50 contig size of 220.4 kb and only 2.96% gaps. A BUSCO analysis indicates that 97.7% of the single-copy Tetrapoda orthologs were recovered in the assembly. In total, 19,829 gene models were annotated to the genome using a combination of ab initio and homology-based methods. To improve the chromosome-level assembly, we generated a high-density linkage map from wild-caught families and developed a novel analytical pipeline to accommodate multiple paternity and unknown father genotypes. We successfully anchored and oriented almost 90% of the genome on 19 linkage groups. This annotated and oriented chromosome-level reference genome represents a valuable resource to facilitate evolutionary studies in squamate reptiles.
Original languageEnglish
Pages (from-to)1953-1960
Number of pages8
JournalGenome Biology and Evolution
Volume12
Issue number11
DOIs
Publication statusPublished - 24 Aug 2020

Fingerprint

Dive into the research topics of 'Chromosome-level assembly of the common lizard (zootoca vivipara) genome'. Together they form a unique fingerprint.

Cite this