Circular dichroism analysis of insect cell expressed herpes simplex virus type I single-stranded DNA-binding protein ICP8

M. Spatz; S.A. Ali; M.M. Eibl; A. Steinkasserer; M. Auer; C. Graf

doi:10.1006/prep.1999.1046

Circular dichroism analysis of insect cell expressed herpes simplex virus type I single-stranded DNA-binding protein ICP8

M. Spatz, S.A. Ali, M.M. Eibl, A. Steinkasserer, M. Auer, C. Graf

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

We have developed a baculovirus expression system for the rapid and efficient production of large quantities (> 5 rag/10 cells) of ICP8. The recombinant ICP8 is fully functional and binds to single-stranded DNA. Secondary structure calculations from circular dichroism measurements indicate a content of 34.5% α-helix and 15.4% β-sheet. This is the first structural report for ICP8 using CD analysis, which will be very useful for high-throughput assay development and mechanistic studies.

Original language	English
Pages (from-to)	40-46
Number of pages	7
Journal	Protein Expression and Purification
Volume	16
Issue number	1
DOIs	https://doi.org/10.1006/prep.1999.1046
Publication status	Published - 1 Jun 1999

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10.1006/prep.1999.1046

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AU - Auer, M.

AU - Graf, C.

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Circular dichroism analysis of insect cell expressed herpes simplex virus type I single-stranded DNA-binding protein ICP8

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