Circular dichroism analysis of insect cell expressed herpes simplex virus type I single-stranded DNA-binding protein ICP8

M. Spatz, S.A. Ali, M.M. Eibl, A. Steinkasserer, M. Auer, C. Graf

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We have developed a baculovirus expression system for the rapid and efficient production of large quantities (> 5 rag/10 cells) of ICP8. The recombinant ICP8 is fully functional and binds to single-stranded DNA. Secondary structure calculations from circular dichroism measurements indicate a content of 34.5% α-helix and 15.4% β-sheet. This is the first structural report for ICP8 using CD analysis, which will be very useful for high-throughput assay development and mechanistic studies.
Original languageEnglish
Pages (from-to)40-46
Number of pages7
JournalProtein Expression and Purification
Volume16
Issue number1
DOIs
Publication statusPublished - 1 Jun 1999

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