Circumsporozoite gene of plasmodium cynomolgi (Gombak): cDNA cloning and expression of the repetitive circumsporozoite epitope

V ENEA, D ARNOT, E C SCHMIDT, A COCHRANE, R GWADZ, R S NUSSENZWEIG

Research output: Contribution to journalArticlepeer-review

Abstract

We report the identification, sequence, and expression in Escherichia coli of the immunodominant epitope of the circumsporozoite (CS) gene of Plasmodium cynomolgi (Gombak), a simian malaria parasite. This epitope is encoded by a DNA sequence that is tandemly repeated 10 times in the cDNA clone. Subclones that contain and express only repeats and in variable number have been constructed. We show that the binding of a specific anti-CS protein monoclonal antibody correlates positively with the number of repeats in each subclone. The CS gene of another strain of P. cynomolgi (NIH) encodes an immunodominant epitope that is immunologically distinct from that of the Gombak strain. We present evidence that these two CS genes share extensive overall homology, although the nucleotide sequences that encode the epitopes appear to be unrelated.
Original languageEnglish
Pages (from-to)7520-7524
Number of pages5
JournalProceedings of the National Academy of Sciences
Volume81
Issue number23
Publication statusPublished - 1984

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