Clinical evaluation of a single reaction, diagnostic polymerase chain reaction assay for the detection of hepatitis C virus RNA

G Gerken, P Pontisso, M Roggendorf, M Grazia Rumi, P Simmonds, C Trepo, S Zeuzem, G Colucci

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

In the past few years the detection of HCV-RNA by polymerase chain reaction has become a well-established diagnostic tool for patients with chronic hepatitis C. However, the lack of reproducible results between laboratories and the relatively high proportion of false-positive results, has indicated the need for a standardized and reliable polymerase chain reaction assay. In the present study we have analyzed the performance of a commercial, HCV-RNA polymerase chain reaction assay based on a single, combined reverse transcription and amplification reaction and on the use of Uracil-N-glycosilase to prevent carry-over contamination (Amplicor HCV, Roche Molecular Systems).
Original languageEnglish
Pages (from-to)33-7
Number of pages5
JournalJournal of Hepatology
Volume24
Issue number1
Publication statusPublished - Jan 1996

Keywords / Materials (for Non-textual outputs)

  • Hepacivirus
  • Hepatitis C
  • Hepatitis C Antibodies
  • Humans
  • Polymerase Chain Reaction
  • RNA, Viral

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