Clinical validity of plasma and urinary desmosine as biomarkers for chronic obstructive pulmonary disease

Jeffrey T-J Huang; Rekha Chaudhuri; Osama Albarbarawi; Alun Barton; Christal Grierson; Petra Rauchhaus; Christopher J. Weir; Martina Messow; Nicola Stevens; Charles McSharry; Giora Feuerstein; Somnath Mukhopadhyay; Jeffrey Brady; Colin N. A. Palmer; Douglas Miller; Neil C. Thomson

doi:10.1136/thoraxjnl-2011-200279

Clinical validity of plasma and urinary desmosine as biomarkers for chronic obstructive pulmonary disease

Jeffrey T-J Huang, Rekha Chaudhuri, Osama Albarbarawi, Alun Barton, Christal Grierson, Petra Rauchhaus, Christopher J. Weir, Martina Messow, Nicola Stevens, Charles McSharry, Giora Feuerstein, Somnath Mukhopadhyay, Jeffrey Brady, Colin N. A. Palmer, Douglas Miller, Neil C. Thomson

Deanery of Molecular, Genetic and Population Health Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Background Although an increased concentration of degraded elastin products in patients with chronic obstructive pulmonary disease (CORD) has been reported for many years, its clinical validity and utility remain uncertain due to technical difficulties, small study groups and the unknown relationship between exacerbation and elastin degradation. The objectives of this study were to determine the validity of urinary and blood total desmosine/isodesmosine in patients with CORD and asthma and to evaluate their relationship to exacerbation status and lung function.

Methods Urinary and blood desmosine levels were measured using validated isotopic dilution liquid chromatography tandem mass spectrometry methods.

Results 390 study participants were recruited from the following groups: healthy volunteers, stable asthma, stable and 'during an exacerbation' CORD. Compared with healthy non-smokers, we found increased urinary or blood desmosine levels in patients with CORD, but no differences in patients with asthma or healthy smokers. The elevation of urinary desmosine levels was associated with the exacerbation status in patients with CORD. Approximately 40% of patients with stable and 'during an exacerbation' CORD showed elevated blood desmosine levels. Blood desmosine levels were strongly associated with age and were negatively correlated with lung diffusing capacity for carbon monoxide.

Conclusion The results suggest that urinary desmosine levels are raised by exacerbations of CORD whereas blood desmosine levels are elevated in a subgroup of patients with stable CORD and reduced lung diffusing capacity. The authors speculate that a raised blood desmosine level may identify patients with increased elastin degradation suitable for targeted therapy. Future prospective studies are required to investigate this hypothesis.

Original language	English
Pages (from-to)	502-508
Number of pages	7
Journal	Thorax
Volume	67
Issue number	6
DOIs	https://doi.org/10.1136/thoraxjnl-2011-200279
Publication status	Published - Jun 2012

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10.1136/thoraxjnl-2011-200279

Clinical validity of plasma and urinary desmosine as biomarkers for chronic obstructive pulmonary disease
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http://thorax.bmj.com/content/67/6/502

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Huang, J. T-J., Chaudhuri, R., Albarbarawi, O., Barton, A., Grierson, C., Rauchhaus, P., Weir, C. J., Messow, M., Stevens, N., McSharry, C., Feuerstein, G., Mukhopadhyay, S., Brady, J., Palmer, C. N. A., Miller, D., & Thomson, N. C. (2012). Clinical validity of plasma and urinary desmosine as biomarkers for chronic obstructive pulmonary disease. Thorax, 67(6), 502-508. https://doi.org/10.1136/thoraxjnl-2011-200279

@article{eb6b2236a1c34170960dcdebdc8e0ad5,

title = "Clinical validity of plasma and urinary desmosine as biomarkers for chronic obstructive pulmonary disease",

abstract = "Background Although an increased concentration of degraded elastin products in patients with chronic obstructive pulmonary disease (CORD) has been reported for many years, its clinical validity and utility remain uncertain due to technical difficulties, small study groups and the unknown relationship between exacerbation and elastin degradation. The objectives of this study were to determine the validity of urinary and blood total desmosine/isodesmosine in patients with CORD and asthma and to evaluate their relationship to exacerbation status and lung function.Methods Urinary and blood desmosine levels were measured using validated isotopic dilution liquid chromatography tandem mass spectrometry methods.Results 390 study participants were recruited from the following groups: healthy volunteers, stable asthma, stable and 'during an exacerbation' CORD. Compared with healthy non-smokers, we found increased urinary or blood desmosine levels in patients with CORD, but no differences in patients with asthma or healthy smokers. The elevation of urinary desmosine levels was associated with the exacerbation status in patients with CORD. Approximately 40% of patients with stable and 'during an exacerbation' CORD showed elevated blood desmosine levels. Blood desmosine levels were strongly associated with age and were negatively correlated with lung diffusing capacity for carbon monoxide.Conclusion The results suggest that urinary desmosine levels are raised by exacerbations of CORD whereas blood desmosine levels are elevated in a subgroup of patients with stable CORD and reduced lung diffusing capacity. The authors speculate that a raised blood desmosine level may identify patients with increased elastin degradation suitable for targeted therapy. Future prospective studies are required to investigate this hypothesis.",

author = "Huang, {Jeffrey T-J} and Rekha Chaudhuri and Osama Albarbarawi and Alun Barton and Christal Grierson and Petra Rauchhaus and Weir, {Christopher J.} and Martina Messow and Nicola Stevens and Charles McSharry and Giora Feuerstein and Somnath Mukhopadhyay and Jeffrey Brady and Palmer, {Colin N. A.} and Douglas Miller and Thomson, {Neil C.}",

year = "2012",

month = jun,

doi = "10.1136/thoraxjnl-2011-200279",

language = "English",

volume = "67",

pages = "502--508",

journal = "Thorax",

issn = "0040-6376",

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Huang, JT-J, Chaudhuri, R, Albarbarawi, O, Barton, A, Grierson, C, Rauchhaus, P, Weir, CJ, Messow, M, Stevens, N, McSharry, C, Feuerstein, G, Mukhopadhyay, S, Brady, J, Palmer, CNA, Miller, D & Thomson, NC 2012, 'Clinical validity of plasma and urinary desmosine as biomarkers for chronic obstructive pulmonary disease', Thorax, vol. 67, no. 6, pp. 502-508. https://doi.org/10.1136/thoraxjnl-2011-200279

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T1 - Clinical validity of plasma and urinary desmosine as biomarkers for chronic obstructive pulmonary disease

AU - Huang, Jeffrey T-J

AU - Chaudhuri, Rekha

AU - Albarbarawi, Osama

AU - Barton, Alun

AU - Grierson, Christal

AU - Rauchhaus, Petra

AU - Weir, Christopher J.

AU - Messow, Martina

AU - Stevens, Nicola

AU - McSharry, Charles

AU - Feuerstein, Giora

AU - Mukhopadhyay, Somnath

AU - Brady, Jeffrey

AU - Palmer, Colin N. A.

AU - Miller, Douglas

AU - Thomson, Neil C.

PY - 2012/6

Y1 - 2012/6

N2 - Background Although an increased concentration of degraded elastin products in patients with chronic obstructive pulmonary disease (CORD) has been reported for many years, its clinical validity and utility remain uncertain due to technical difficulties, small study groups and the unknown relationship between exacerbation and elastin degradation. The objectives of this study were to determine the validity of urinary and blood total desmosine/isodesmosine in patients with CORD and asthma and to evaluate their relationship to exacerbation status and lung function.Methods Urinary and blood desmosine levels were measured using validated isotopic dilution liquid chromatography tandem mass spectrometry methods.Results 390 study participants were recruited from the following groups: healthy volunteers, stable asthma, stable and 'during an exacerbation' CORD. Compared with healthy non-smokers, we found increased urinary or blood desmosine levels in patients with CORD, but no differences in patients with asthma or healthy smokers. The elevation of urinary desmosine levels was associated with the exacerbation status in patients with CORD. Approximately 40% of patients with stable and 'during an exacerbation' CORD showed elevated blood desmosine levels. Blood desmosine levels were strongly associated with age and were negatively correlated with lung diffusing capacity for carbon monoxide.Conclusion The results suggest that urinary desmosine levels are raised by exacerbations of CORD whereas blood desmosine levels are elevated in a subgroup of patients with stable CORD and reduced lung diffusing capacity. The authors speculate that a raised blood desmosine level may identify patients with increased elastin degradation suitable for targeted therapy. Future prospective studies are required to investigate this hypothesis.

AB - Background Although an increased concentration of degraded elastin products in patients with chronic obstructive pulmonary disease (CORD) has been reported for many years, its clinical validity and utility remain uncertain due to technical difficulties, small study groups and the unknown relationship between exacerbation and elastin degradation. The objectives of this study were to determine the validity of urinary and blood total desmosine/isodesmosine in patients with CORD and asthma and to evaluate their relationship to exacerbation status and lung function.Methods Urinary and blood desmosine levels were measured using validated isotopic dilution liquid chromatography tandem mass spectrometry methods.Results 390 study participants were recruited from the following groups: healthy volunteers, stable asthma, stable and 'during an exacerbation' CORD. Compared with healthy non-smokers, we found increased urinary or blood desmosine levels in patients with CORD, but no differences in patients with asthma or healthy smokers. The elevation of urinary desmosine levels was associated with the exacerbation status in patients with CORD. Approximately 40% of patients with stable and 'during an exacerbation' CORD showed elevated blood desmosine levels. Blood desmosine levels were strongly associated with age and were negatively correlated with lung diffusing capacity for carbon monoxide.Conclusion The results suggest that urinary desmosine levels are raised by exacerbations of CORD whereas blood desmosine levels are elevated in a subgroup of patients with stable CORD and reduced lung diffusing capacity. The authors speculate that a raised blood desmosine level may identify patients with increased elastin degradation suitable for targeted therapy. Future prospective studies are required to investigate this hypothesis.

U2 - 10.1136/thoraxjnl-2011-200279

DO - 10.1136/thoraxjnl-2011-200279

M3 - Article

VL - 67

SP - 502

EP - 508

JO - Thorax

JF - Thorax

SN - 0040-6376

IS - 6

ER -

Clinical validity of plasma and urinary desmosine as biomarkers for chronic obstructive pulmonary disease

Abstract

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