Clonal analysis of T cell responses to herpes simplex virus: a HSV-specific, non cytolytic T cell clone displays specific helper activity for T cell response in vitro.

K.N Leung; Anthony Nash

doi:10.1016/0165-2478(86)90126-4

Clonal analysis of T cell responses to herpes simplex virus: a HSV-specific, non cytolytic T cell clone displays specific helper activity for T cell response in vitro.

K.N Leung, Anthony Nash

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

A HSV-specific, Lyt 1+2- T cell clone (D7.1) was established by repetitive in vitro restimulation of in vivo HSV-1 primed draining lymph node cells with UV-inactivated homologous virus. Earlier work had shown that this clone could provide specific helper activity for HSV-1 primed B cells for antibody production in vivo. In this paper, further functional analysis has demonstrated that this clone and its subclones could also help unprimed T cells in the generation of effector DTH T cells in vitro. The helper activity was found to be antigen-specific and cell dose-dependent. In addition, this helper T cell clone showed little, if any, natural killer-like activity and was non-cytolytic towards both HSV-infected and uninfected syngeneic targets. However, they could exert potent cytotoxic activity towards P815 and EL-4 tumour targets in the presence of lectins.

Original language	English
Pages (from-to)	55-61
Number of pages	7
Journal	Immunology Letters
Volume	13
Issue number	1-2
DOIs	https://doi.org/10.1016/0165-2478(86)90126-4
Publication status	Published - Aug 1986

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title = "Clonal analysis of T cell responses to herpes simplex virus: a HSV-specific, non cytolytic T cell clone displays specific helper activity for T cell response in vitro.",

abstract = "A HSV-specific, Lyt 1+2- T cell clone (D7.1) was established by repetitive in vitro restimulation of in vivo HSV-1 primed draining lymph node cells with UV-inactivated homologous virus. Earlier work had shown that this clone could provide specific helper activity for HSV-1 primed B cells for antibody production in vivo. In this paper, further functional analysis has demonstrated that this clone and its subclones could also help unprimed T cells in the generation of effector DTH T cells in vitro. The helper activity was found to be antigen-specific and cell dose-dependent. In addition, this helper T cell clone showed little, if any, natural killer-like activity and was non-cytolytic towards both HSV-infected and uninfected syngeneic targets. However, they could exert potent cytotoxic activity towards P815 and EL-4 tumour targets in the presence of lectins.",

author = "K.N Leung and Anthony Nash",

year = "1986",

month = aug,

doi = "10.1016/0165-2478(86)90126-4",

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journal = "Immunology Letters",

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T1 - Clonal analysis of T cell responses to herpes simplex virus: a HSV-specific, non cytolytic T cell clone displays specific helper activity for T cell response in vitro.

AU - Leung, K.N

AU - Nash, Anthony

PY - 1986/8

Y1 - 1986/8

N2 - A HSV-specific, Lyt 1+2- T cell clone (D7.1) was established by repetitive in vitro restimulation of in vivo HSV-1 primed draining lymph node cells with UV-inactivated homologous virus. Earlier work had shown that this clone could provide specific helper activity for HSV-1 primed B cells for antibody production in vivo. In this paper, further functional analysis has demonstrated that this clone and its subclones could also help unprimed T cells in the generation of effector DTH T cells in vitro. The helper activity was found to be antigen-specific and cell dose-dependent. In addition, this helper T cell clone showed little, if any, natural killer-like activity and was non-cytolytic towards both HSV-infected and uninfected syngeneic targets. However, they could exert potent cytotoxic activity towards P815 and EL-4 tumour targets in the presence of lectins.

AB - A HSV-specific, Lyt 1+2- T cell clone (D7.1) was established by repetitive in vitro restimulation of in vivo HSV-1 primed draining lymph node cells with UV-inactivated homologous virus. Earlier work had shown that this clone could provide specific helper activity for HSV-1 primed B cells for antibody production in vivo. In this paper, further functional analysis has demonstrated that this clone and its subclones could also help unprimed T cells in the generation of effector DTH T cells in vitro. The helper activity was found to be antigen-specific and cell dose-dependent. In addition, this helper T cell clone showed little, if any, natural killer-like activity and was non-cytolytic towards both HSV-infected and uninfected syngeneic targets. However, they could exert potent cytotoxic activity towards P815 and EL-4 tumour targets in the presence of lectins.

U2 - 10.1016/0165-2478(86)90126-4

DO - 10.1016/0165-2478(86)90126-4

M3 - Article

SN - 0165-2478

VL - 13

SP - 55

EP - 61

JO - Immunology Letters

JF - Immunology Letters

IS - 1-2

ER -

Clonal analysis of T cell responses to herpes simplex virus: a HSV-specific, non cytolytic T cell clone displays specific helper activity for T cell response in vitro.

Abstract

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