Clonal analysis of T-cell responses to herpes simplex virus: isolation, characterization and antiviral properties of an antigen-specific helper T-cell clone

K.N Leung, Anthony Nash, D Y Sia, P Wildy

Research output: Contribution to journalArticlepeer-review

Abstract

A herpes simplex virus (HSV)-specific long-term T-cell clone has been established from the draining lymph node cells of BALB/c mice; the cells required repeated in vitro restimulation with UV-irradiated virus. The established T-cell clone expresses the Thy-1 and Lyt-1+2,3- surface antigens. For optimal proliferation of the cloned cells, both the presence of specific antigen and an exogenous source of T-cell growth factor are required. The proliferative response of the cloned T cells was found to be virus-specific but it did not distinguish between HSV-1 and HSV-2. Adoptive cell transfer of the cloned T cells helped primed B cells to produce anti-herpes antibodies: the response was antigen-specific and cell dose-dependent. The clone failed to produce a significant DTH reaction in vivo, but did produce high levels of macrophage-activating factor. Furthermore, the T-cell clone could protect from HSV infection, as measured by a reduction in local virus growth, and by enhanced survival following the challenge of mice with a lethal dose of virus. The mechanism(s) whereby this clone protects in vivo is discussed.
Original languageEnglish
Pages (from-to)623-33
Number of pages11
JournalImmunology
Volume53
Issue number4
Publication statusPublished - Dec 1984

Keywords

  • Animals
  • Antibodies, Viral/biosynthesis
  • Antigens, Surface/analysis
  • Clone Cells/immunology
  • Epitopes/immunology
  • Female
  • Herpes Simplex/immunology
  • Hypersensitivity, Delayed
  • Immunization, Passive
  • Interleukin-2/immunology
  • Lymphocyte Activation
  • Lymphocyte Cooperation
  • Lymphokines/biosynthesis
  • Macrophage-Activating Factors
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred Strains
  • Simplexvirus/immunology
  • T-Lymphocytes, Helper-Inducer/immunology

Fingerprint

Dive into the research topics of 'Clonal analysis of T-cell responses to herpes simplex virus: isolation, characterization and antiviral properties of an antigen-specific helper T-cell clone'. Together they form a unique fingerprint.

Cite this