Cloning and expression of feline colony stimulating factor receptor (CSF-1R) and analysis of the species specificity of stimulation by colony stimulating factor-1 (CSF-1) and interleukin-34 (IL-34).

Deborah J. Gow, Valerie Garceau, Clare Pridans, Adam Gow, K. E. Simpson, Danielle Gunn-Moore, David Hume

Research output: Contribution to journalArticlepeer-review

Abstract

Colony stimulating factor (CSF-1) and its receptor, CSF-1R, have been previously well studied in humans and rodents to dissect the role they play in development of cells of the mononuclear phagocyte system. A second ligand for the CSF-1R, IL-34 has been described in several species. In this study, we have cloned and expressed the feline CSF-1R and examined the responsiveness to CSF-1 and IL-34 from a range of species. The results indicate that pig and human CSF-1 and human IL-34 are equally effective in cats, where both mouse CSF-1 and IL-34 are significantly less active. Recombinant human CSF-1 can be used to generate populations of feline bone marrow and monocyte derived macrophages that can be used to further dissect macrophage-specific gene expression in this species, and to compare it to data derived from mouse, human and pig. These results set the scene for therapeutic use of CSF-1 and IL-34 in cats.
Original languageEnglish
Pages (from-to)630-638
Number of pages9
JournalCytokine
Volume61
Issue number2
Early online date17 Dec 2012
DOIs
Publication statusPublished - 2013

Keywords

  • Macrophage
  • Ba/F3
  • Bone marrow
  • Species specificity
  • Renal

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