Cloning and mapping of the porcine cytochrome-p450 2E1 gene and its association with skatole levels in the domestic pig

T M Skinner; E Doran; J D McGivan; C S Haley; A L Archibald

doi:10.1111/j.1365-2052.2005.01342.x

Cloning and mapping of the porcine cytochrome-p450 2E1 gene and its association with skatole levels in the domestic pig

T M Skinner, E Doran, J D McGivan, C S Haley, A L Archibald

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The porcine cytochrome-p450 2E1 (CYP2E1) gene was isolated by screening a pig BAC library and partially sequenced. This sequence information was used to identify six single nucleotide polymorphisms (SNPs) within the CYP2E1 gene and its promoter. In addition, a microsatellite marker tightly linked to the CYP2E1 gene was subcloned from the BAC. One of these markers was used to map the CYP2E1 gene distal of SWC27 on SSC14, well outside reported quantitative trait loci on SSC14 for skatole, indole and taste test measures of boar taint. However, in a population of commercial pigs scored for backfat skatole levels, there was evidence of association between a SNP in the CYP2E1 promoter and skatole deposition, although there was no significant association between this SNP and skatole levels in the experimental cross.

Original language	English
Pages (from-to)	417-22
Number of pages	6
Journal	Animal Genetics
Volume	36
Issue number	5
DOIs	https://doi.org/10.1111/j.1365-2052.2005.01342.x
Publication status	Published - Oct 2005

Keywords / Materials (for Non-textual outputs)

Adipose Tissue
Animals
Base Sequence
Chromosome Mapping
Chromosomes, Artificial, Bacterial
Cloning, Molecular
Crosses, Genetic
Cytochrome P-450 CYP2E1
DNA Primers
Gene Frequency
Microsatellite Repeats
Molecular Sequence Data
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Sequence Analysis, DNA
Skatole
Sus scrofa

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10.1111/j.1365-2052.2005.01342.x

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title = "Cloning and mapping of the porcine cytochrome-p450 2E1 gene and its association with skatole levels in the domestic pig",

abstract = "The porcine cytochrome-p450 2E1 (CYP2E1) gene was isolated by screening a pig BAC library and partially sequenced. This sequence information was used to identify six single nucleotide polymorphisms (SNPs) within the CYP2E1 gene and its promoter. In addition, a microsatellite marker tightly linked to the CYP2E1 gene was subcloned from the BAC. One of these markers was used to map the CYP2E1 gene distal of SWC27 on SSC14, well outside reported quantitative trait loci on SSC14 for skatole, indole and taste test measures of boar taint. However, in a population of commercial pigs scored for backfat skatole levels, there was evidence of association between a SNP in the CYP2E1 promoter and skatole deposition, although there was no significant association between this SNP and skatole levels in the experimental cross.",

keywords = "Adipose Tissue, Animals, Base Sequence, Chromosome Mapping, Chromosomes, Artificial, Bacterial, Cloning, Molecular, Crosses, Genetic, Cytochrome P-450 CYP2E1, DNA Primers, Gene Frequency, Microsatellite Repeats, Molecular Sequence Data, Polymorphism, Restriction Fragment Length, Polymorphism, Single Nucleotide, Sequence Analysis, DNA, Skatole, Sus scrofa",

author = "Skinner, {T M} and E Doran and McGivan, {J D} and Haley, {C S} and Archibald, {A L}",

year = "2005",

month = oct,

doi = "10.1111/j.1365-2052.2005.01342.x",

language = "English",

volume = "36",

pages = "417--22",

journal = "Animal Genetics",

issn = "0268-9146",

publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Cloning and mapping of the porcine cytochrome-p450 2E1 gene and its association with skatole levels in the domestic pig

AU - Skinner, T M

AU - Doran, E

AU - McGivan, J D

AU - Haley, C S

AU - Archibald, A L

PY - 2005/10

Y1 - 2005/10

N2 - The porcine cytochrome-p450 2E1 (CYP2E1) gene was isolated by screening a pig BAC library and partially sequenced. This sequence information was used to identify six single nucleotide polymorphisms (SNPs) within the CYP2E1 gene and its promoter. In addition, a microsatellite marker tightly linked to the CYP2E1 gene was subcloned from the BAC. One of these markers was used to map the CYP2E1 gene distal of SWC27 on SSC14, well outside reported quantitative trait loci on SSC14 for skatole, indole and taste test measures of boar taint. However, in a population of commercial pigs scored for backfat skatole levels, there was evidence of association between a SNP in the CYP2E1 promoter and skatole deposition, although there was no significant association between this SNP and skatole levels in the experimental cross.

AB - The porcine cytochrome-p450 2E1 (CYP2E1) gene was isolated by screening a pig BAC library and partially sequenced. This sequence information was used to identify six single nucleotide polymorphisms (SNPs) within the CYP2E1 gene and its promoter. In addition, a microsatellite marker tightly linked to the CYP2E1 gene was subcloned from the BAC. One of these markers was used to map the CYP2E1 gene distal of SWC27 on SSC14, well outside reported quantitative trait loci on SSC14 for skatole, indole and taste test measures of boar taint. However, in a population of commercial pigs scored for backfat skatole levels, there was evidence of association between a SNP in the CYP2E1 promoter and skatole deposition, although there was no significant association between this SNP and skatole levels in the experimental cross.

KW - Adipose Tissue

KW - Animals

KW - Base Sequence

KW - Chromosome Mapping

KW - Chromosomes, Artificial, Bacterial

KW - Cloning, Molecular

KW - Crosses, Genetic

KW - Cytochrome P-450 CYP2E1

KW - DNA Primers

KW - Gene Frequency

KW - Microsatellite Repeats

KW - Molecular Sequence Data

KW - Polymorphism, Restriction Fragment Length

KW - Polymorphism, Single Nucleotide

KW - Sequence Analysis, DNA

KW - Skatole

KW - Sus scrofa

U2 - 10.1111/j.1365-2052.2005.01342.x

DO - 10.1111/j.1365-2052.2005.01342.x

M3 - Article

C2 - 16167985

SN - 0268-9146

VL - 36

SP - 417

EP - 422

JO - Animal Genetics

JF - Animal Genetics

IS - 5

ER -

Cloning and mapping of the porcine cytochrome-p450 2E1 gene and its association with skatole levels in the domestic pig

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Keywords / Materials (for Non-textual outputs)

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