Cloning and sequence analysis of cDNA for corticotropin-releasing factor precursor from the teleost fish Catostomus commersoni

Y Okawara, S D Morley, L O Burzio, H Zwiers, K Lederis, D Richter

Research output: Contribution to journalArticlepeer-review

Abstract

The sequence of a cDNA encoding the corticotropin-releasing factor precursor has been identified by screening lambda gt11 libraries constructed from poly(A)+ RNA of the hypothalamic region of the white sucker Catostomus commersoni brain with synthetic oligonucleotide probes deduced from the sequence of the rat corticotropin-releasing factor. The amino acid sequence of corticotropin-releasing factor of the sucker is strikingly conserved when compared to its counterpart from rat and differs only in two positions at the carboxyl terminus; in contrast, there is little similarity between their cryptic regions.

Original languageEnglish
Pages (from-to)8439-43
Number of pages5
JournalProceedings of the National Academy of Sciences
Volume85
Issue number22
Publication statusPublished - Nov 1988

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Codon
  • DNA
  • Fishes
  • Genes
  • Molecular Sequence Data
  • Restriction Mapping

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