Cloning and sequencing of feline and canine ice-related cDNAs encoding hybrid caspase-1/caspase-13-like propeptides

S Taylor, L Hanlon, C McGillivray, E A Gault, D J Argyle, D E Onions, L Nicolson

Research output: Contribution to journalArticlepeer-review

Abstract

Caspases are cysteine proteases which have important roles in the activation of cytokines and in apoptosis. The ICE subfamily of caspases comprise peptides closely related to caspase-1, or interleukin-1beta (IL-1beta) converting enzyme (ICE), which promotes maturation of interleukin IL-1beta and interleukin-18 (IL-18) by proteolytic cleavage of precursor forms to generate biologically active peptides. Other members of this subfamily include caspase-4, -5, -13 and isoforms of these proteins. We report the cloning and sequencing of two feline and canine ICE-related cDNAs amplified by RT-PCR. The predicted proteins are 410 and 404 amino acids in length respectively and are most closely related to caspase-1 sequences across the N-terminal 115 amino acids and to human caspase-13 across the C-terminal sequence.
Original languageEnglish
Pages (from-to)387-94
Number of pages8
JournalDNA Sequence
Volume10
Issue number6
Publication statusPublished - 2000

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Caspase 1
  • Caspases
  • Cats
  • Cloning, Molecular
  • DNA, Complementary
  • Dogs
  • Molecular Sequence Data
  • Protein Precursors
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

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